Macro-microscopic fluorescence of human bladder cancer using hypericin fluorescence cystoscopy and laser confocal microscopy

  • Authors:
    • Malini Olivo
    • Weber Lau
    • Vanaja Manivasager
    • Puay Hoon Tan
    • Khee Chee Soo
    • Christopher Cheng
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  • Published online on: October 1, 2003     https://doi.org/10.3892/ijo.23.4.983
  • Pages: 983-990
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Abstract

The early detection of carcinoma is very essential for the diagnosis and prognosis of a bladder cancer patient. In this study we have investigated the use of hypericin as a fluorescent tumour marker and laser confocal microscopy as a diagnostic tool to aid the diagnosis of such cancers. Both cellular and clinical studies have been conducted. In the cellular studies, we have compared two bladder cell lines for the uptake and sub-cellular localization of hypericin. It was found that there was a rapid uptake and clearance of hypericin and significant localization in mitochondria and lysosomes. The study also revealed that there was a time-dependent increase in fluorescence intensity in bladder cells. The optimum localization was found to be 2-4 h post drug incubation. In the clinical study, consisting of 30 patients, both white light and fluorescence cystoscopy were performed after hypericin instillation. Biopsies taken from fluorescing regions were imaged using the confocal microscope. The order of fluorescence was detected to be as follows: normal < inflammation < grade 1 < grade 2 < CIS < grade 3. It was also found that the fluorescence intensity increased with the stage of the disease thereby enabling the determination of the degree of invasiveness of cancer. This enables the use of hypericin as a prognostic marker and laser confocal microscopy as a tool to aid in diagnosis of bladder cancer.

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October 2003
Volume 23 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Olivo M, Lau W, Manivasager V, Tan PH, Soo KC and Cheng C: Macro-microscopic fluorescence of human bladder cancer using hypericin fluorescence cystoscopy and laser confocal microscopy. Int J Oncol 23: 983-990, 2003
APA
Olivo, M., Lau, W., Manivasager, V., Tan, P.H., Soo, K.C., & Cheng, C. (2003). Macro-microscopic fluorescence of human bladder cancer using hypericin fluorescence cystoscopy and laser confocal microscopy. International Journal of Oncology, 23, 983-990. https://doi.org/10.3892/ijo.23.4.983
MLA
Olivo, M., Lau, W., Manivasager, V., Tan, P. H., Soo, K. C., Cheng, C."Macro-microscopic fluorescence of human bladder cancer using hypericin fluorescence cystoscopy and laser confocal microscopy". International Journal of Oncology 23.4 (2003): 983-990.
Chicago
Olivo, M., Lau, W., Manivasager, V., Tan, P. H., Soo, K. C., Cheng, C."Macro-microscopic fluorescence of human bladder cancer using hypericin fluorescence cystoscopy and laser confocal microscopy". International Journal of Oncology 23, no. 4 (2003): 983-990. https://doi.org/10.3892/ijo.23.4.983