Eicosapentaenoic acid perturbs signalling via the NFκB transcriptional pathway in pancreatic tumour cells

  • Authors:
    • James A. Ross
    • Jean P. Maingay
    • Kenneth C.H. Fearon
    • Kathryn Sangster
    • James J. Powell
  • View Affiliations

  • Published online on: December 1, 2003     https://doi.org/10.3892/ijo.23.6.1733
  • Pages: 1733-1738
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

In addition to various roles in membrane structure and metabolism, polyunsaturated fatty acids have effects on signal transduction and on the regulation of gene expression. Eicosapentaenoic acid (EPA) is an ω-3 fatty acid which is known to induce cell cycle arrest and apoptosis in pancreatic tumour cells. NFκB is a key transcription factor regulating genes involved in the immune response and has been implicated in apoptotic pathways. In this study we investigated the effect of eicosapentanoic acid on the NFκB pathway in pancreatic tumour cells. The pancreatic cell line MIA PaCa2 was incubated in the presence of the fatty acids EPA (n-3), arachidonic acid (AA, n-6) or oleic acid (OA, n-9) before pulsing with TNF to provide a kinetic assessment of NFκB activation and IκBα degradation. Pre-incubation of pancreatic cells with EPA or AA for 2 h before pulsing with TNF preserved IκBα but did not prevent NFκB activation. Indeed, NFκB activation was prolonged after exposure to EPA. N-acetyl-L-cysteine did not influence the effect of EPA on TNF-stimulated IκBα degradation. These results suggest that the ω-3 fatty acid EPA perturbs the NFκB pathway by a novel mechanism. This mechanism may be important in delineating alternative pathways to NFκB activation.

Related Articles

Journal Cover

December 2003
Volume 23 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Ross JA, Maingay JP, Fearon KC, Sangster K and Powell JJ: Eicosapentaenoic acid perturbs signalling via the NFκB transcriptional pathway in pancreatic tumour cells. Int J Oncol 23: 1733-1738, 2003.
APA
Ross, J.A., Maingay, J.P., Fearon, K.C., Sangster, K., & Powell, J.J. (2003). Eicosapentaenoic acid perturbs signalling via the NFκB transcriptional pathway in pancreatic tumour cells. International Journal of Oncology, 23, 1733-1738. https://doi.org/10.3892/ijo.23.6.1733
MLA
Ross, J. A., Maingay, J. P., Fearon, K. C., Sangster, K., Powell, J. J."Eicosapentaenoic acid perturbs signalling via the NFκB transcriptional pathway in pancreatic tumour cells". International Journal of Oncology 23.6 (2003): 1733-1738.
Chicago
Ross, J. A., Maingay, J. P., Fearon, K. C., Sangster, K., Powell, J. J."Eicosapentaenoic acid perturbs signalling via the NFκB transcriptional pathway in pancreatic tumour cells". International Journal of Oncology 23, no. 6 (2003): 1733-1738. https://doi.org/10.3892/ijo.23.6.1733