Characteristics of a potent tumor vaccine-induced secondary anti-tumor T cell response

  • Authors:
    • Yolanda D. Mahnke
    • Volker Schirrmacher
  • View Affiliations

  • Published online on: June 1, 2004     https://doi.org/10.3892/ijo.24.6.1427
  • Pages: 1427-1434
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Abstract

This study describes a tumor vaccine-induced secondary in vivo T cell response to an immunodominant epitope of β-galactosidase (Gal) as a model tumor-associated antigen. DBA/2 mice are primed with lacZ transfected live ESbL tumor cells (ESbL-Gal) in the ear pinna, a site which had previously been shown to be non-tumorigenic and immunogenic. Intraperitoneal challenge of such mice with tumor vaccine (i.e., 107 irradiation-inactivated ESbL-Gal cells) leads to the production of a powerful CD8+ CTL response and to the establishment of immune memory. Using peptide/MHC-tetrameric complexes, clonal expansion of antigen-specific T cells could be detected during the primary response in bone marrow (BM) and during the secondary response in the peritoneal cavity and BM. The secondary response in the peritoneal cavity involved a >80-fold enrichment of epitope specific CD8+ T cells and the release of various cytokines, including IL-12 and TNF-α. The recruitment and/or expansion of Gal specific T cells within the peritoneal cavity could be inhibited by anti-IL-12 and anti-TNF-α monoclonal antibody (mAb) treatment. Interestingly, the secondary CTL response was inhibited by anti-IL-12 but not by anti-TNF-α mAb. The results characterize a strong systemic CD8+ memory T cell response to a cell bound antigen without the use of adjuvant.

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June 2004
Volume 24 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Mahnke YD and Mahnke YD: Characteristics of a potent tumor vaccine-induced secondary anti-tumor T cell response. Int J Oncol 24: 1427-1434, 2004
APA
Mahnke, Y.D., & Mahnke, Y.D. (2004). Characteristics of a potent tumor vaccine-induced secondary anti-tumor T cell response. International Journal of Oncology, 24, 1427-1434. https://doi.org/10.3892/ijo.24.6.1427
MLA
Mahnke, Y. D., Schirrmacher, V."Characteristics of a potent tumor vaccine-induced secondary anti-tumor T cell response". International Journal of Oncology 24.6 (2004): 1427-1434.
Chicago
Mahnke, Y. D., Schirrmacher, V."Characteristics of a potent tumor vaccine-induced secondary anti-tumor T cell response". International Journal of Oncology 24, no. 6 (2004): 1427-1434. https://doi.org/10.3892/ijo.24.6.1427