Ultraviolet radiation induces release of MIA: A new mechanism for UVR-induced progression of melanoma

  • Authors:
    • David G. Marr
    • Ina Poser
    • Yiqun G. Shellman
    • Anja K. Bosserhoff
    • David A. Norris
  • View Affiliations

  • Published online on: July 1, 2004     https://doi.org/10.3892/ijo.25.1.105
  • Pages: 105-111
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Abstract

MIA is a potent melanoma detachment factor that interferes with cellular adherence by binding to fibronectin and laminin, blocking their interaction with α4β1 and α5β1 integrins. The direct correlation between serum MIA levels of patients with progressing melanomas and tumor load supports a role for MIA as a melanoma progression factor. The goal of this study was to determine the effect of ultraviolet radiation (UVR), activating ras mutation, or loss of p53 function on MIA expression and release from melanoma cells. We previously showed that transfection of a mutant constitutively active ras into the melanoma cell line, WM35, induces a phenotypic change from radial to vertical growth, exhibiting increased proliferation and migration. Here, we report that MIA release was elevated in a ras-transfected cell line. In addition, loss of functional p53, using a dominant negative construct, substantially lowered the level of MIA release compared to control. UVR stimulated release of MIA into the extracellular compartment in both the control and ras-transfected cell lines. In addition, MIA mRNA was increased following UVR in all cell lines tested. By inducing either apoptosis or necrosis, we were able to confirm that MIA protein is not released from cells due to cell death alone. We have identified a transcriptional effect of UVR on MIA expression and have shown that release of MIA protein is dependent upon functional p53. We propose that UVR-induced production and release of MIA may promote the detachment of radial and vertical growth phase melanomas from basement membrane or matrix proteins, serving as a unique progression mechanism for melanoma.

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July 2004
Volume 25 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Marr DG, Poser I, Shellman YG, Bosserhoff AK and Norris DA: Ultraviolet radiation induces release of MIA: A new mechanism for UVR-induced progression of melanoma. Int J Oncol 25: 105-111, 2004
APA
Marr, D.G., Poser, I., Shellman, Y.G., Bosserhoff, A.K., & Norris, D.A. (2004). Ultraviolet radiation induces release of MIA: A new mechanism for UVR-induced progression of melanoma. International Journal of Oncology, 25, 105-111. https://doi.org/10.3892/ijo.25.1.105
MLA
Marr, D. G., Poser, I., Shellman, Y. G., Bosserhoff, A. K., Norris, D. A."Ultraviolet radiation induces release of MIA: A new mechanism for UVR-induced progression of melanoma". International Journal of Oncology 25.1 (2004): 105-111.
Chicago
Marr, D. G., Poser, I., Shellman, Y. G., Bosserhoff, A. K., Norris, D. A."Ultraviolet radiation induces release of MIA: A new mechanism for UVR-induced progression of melanoma". International Journal of Oncology 25, no. 1 (2004): 105-111. https://doi.org/10.3892/ijo.25.1.105