Overexpression of CD105 in rat myoblasts: Role of CD105 in cell attachment, spreading and survival

  • Authors:
    • Baoqiang Guo
    • Paul Rooney
    • Mark Slevin
    • Chenggang Li
    • Sudeep Parameshwar
    • Donghui Liu
    • Pat Kumar
    • Carmelo Bernabeu
    • Shant Kumar
  • View Affiliations

  • Published online on: August 1, 2004     https://doi.org/10.3892/ijo.25.2.285
  • Pages: 285-291
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Abstract

CD105 is a receptor for transforming growth factor-β but it is also considered to be involved in cellular functions such as cell adhesion and migration. Using CD105 transfected rat myoblasts, we have investigated the role of CD105 in cell adhesion, spreading, growth and migration. CD105 transfected myoblasts expressed abundant CD105, which was preferentially located within focal adhesion sites. These cells took on a bipolar morphology whereas mock cells remained polygonal or rounded, and when wounded, CD105 expressing cells realigned their long axis prior to migrating and migrated as a cohort of cells. CD105 expression promoted cellular attachment, spreading, survival and growth in serum-free conditions and each of these parameters could be inhibited by a RGD-containing peptide but not a RAD-containing peptide. Mock-transfected cells could not attach, spread or grow under these conditions. Attachment, spreading and growth in CD105 expressing cells could be promoted by the addition of a monoclonal antibody against CD105. Expression of CD105 resulted in the phosphorylation of JNK1 but had no effect on β1 integrin expression. From this preliminary study, we conclude that in addition to acting as a transforming growth factor-β receptor, CD105 has an important role in cell adhesion, migration and survival.

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August 2004
Volume 25 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Guo B, Rooney P, Slevin M, Li C, Parameshwar S, Liu D, Kumar P, Bernabeu C and Kumar S: Overexpression of CD105 in rat myoblasts: Role of CD105 in cell attachment, spreading and survival. Int J Oncol 25: 285-291, 2004
APA
Guo, B., Rooney, P., Slevin, M., Li, C., Parameshwar, S., Liu, D. ... Kumar, S. (2004). Overexpression of CD105 in rat myoblasts: Role of CD105 in cell attachment, spreading and survival. International Journal of Oncology, 25, 285-291. https://doi.org/10.3892/ijo.25.2.285
MLA
Guo, B., Rooney, P., Slevin, M., Li, C., Parameshwar, S., Liu, D., Kumar, P., Bernabeu, C., Kumar, S."Overexpression of CD105 in rat myoblasts: Role of CD105 in cell attachment, spreading and survival". International Journal of Oncology 25.2 (2004): 285-291.
Chicago
Guo, B., Rooney, P., Slevin, M., Li, C., Parameshwar, S., Liu, D., Kumar, P., Bernabeu, C., Kumar, S."Overexpression of CD105 in rat myoblasts: Role of CD105 in cell attachment, spreading and survival". International Journal of Oncology 25, no. 2 (2004): 285-291. https://doi.org/10.3892/ijo.25.2.285