A novel cysteine protease HeLa DUB-1 responsible for cleaving the ubiquitin in human ovarian cancer cells

  • Authors:
    • Myung-Sun Kim
    • Kyong-Jai Yoo
    • Incheol Kang
    • Hyung Min Chung
    • Kwang-Hyun Baek
  • View Affiliations

  • Published online on: August 1, 2004     https://doi.org/10.3892/ijo.25.2.373
  • Pages: 373-379
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Abstract

The regulation of ubiquitin-mediated protein degradation is becoming important for a number of cellular processes. Human HeLa DUB-1 cDNA, encoding a novel deubiquitinating enzyme, was isolated from ovarian cancer cells. It has 1,647 bp nucleotides and encodes a 548 amino acid polypeptide with the molecular weight of approximately 61 kDa. It contains the highly conserved Cys, Asp (I), His, and Asn/Asp (II) domains characteristic of the ubiquitin-specific processing proteases. Biochemical assay revealed that HeLa DUB-1 has deubiquitinating enzyme activity in vivo and in vitro. Northern blot analysis for HeLa DUB-1 showed the strong expression in human skeletal muscle and pancreas and to some extent in heart, placenta, lung, liver, and kidney. Interestingly, the expression was hardly seen in the brain. Localization study indicates that HeLa DUB-1 proteins are present in both the cytoplasm and nucleus.

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August 2004
Volume 25 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Kim M, Yoo K, Kang I, Chung HM and Baek K: A novel cysteine protease HeLa DUB-1 responsible for cleaving the ubiquitin in human ovarian cancer cells. Int J Oncol 25: 373-379, 2004
APA
Kim, M., Yoo, K., Kang, I., Chung, H.M., & Baek, K. (2004). A novel cysteine protease HeLa DUB-1 responsible for cleaving the ubiquitin in human ovarian cancer cells. International Journal of Oncology, 25, 373-379. https://doi.org/10.3892/ijo.25.2.373
MLA
Kim, M., Yoo, K., Kang, I., Chung, H. M., Baek, K."A novel cysteine protease HeLa DUB-1 responsible for cleaving the ubiquitin in human ovarian cancer cells". International Journal of Oncology 25.2 (2004): 373-379.
Chicago
Kim, M., Yoo, K., Kang, I., Chung, H. M., Baek, K."A novel cysteine protease HeLa DUB-1 responsible for cleaving the ubiquitin in human ovarian cancer cells". International Journal of Oncology 25, no. 2 (2004): 373-379. https://doi.org/10.3892/ijo.25.2.373