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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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September 2004 Volume 25 Issue 3

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Medicine International

An International Open Access Journal Devoted to General Medicine.

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September 2004 Volume 25 Issue 3

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Article

Topoisomerase inhibitors enhance the cytocidal effect of AAV-HSVtk/ganciclovir on head and neck cancer cells

  • Authors:
    • Takeharu Kanazawa
    • Hiroaki Mizukami
    • Hiroshi Nishino
    • Takashi Okada
    • Yutaka Hanazono
    • Akihiro Kume
    • Ken Kitamura
    • Keiichi Ichimura
    • Keiya Ozawa

  • View Affiliations / Copyright

    Affiliations: Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School, Tochigi 329-0498, Japan
  • Pages: 729-735
    |
    Published online on: September 1, 2004
       https://doi.org/10.3892/ijo.25.3.729
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Abstract

Adeno-associated virus (AAV) is a non-pathogenic virus with a single-strand DNA genome. AAV vectors have several unique properties suited for gene therapy applications. However, an obstacle to their application is a low efficiency of transgene expression, mainly due to a limited second-strand synthesis. Previously, we reported that γ-rays enhanced the transduction efficiency and cytocidal effect of AAV vector harboring the herpes simplex virus-thymidine kinase (AAVtk) and ganciclovir (GCV) system. In the present study, we investigated whether topoisomerase inhibitors (etoposide and camptothecin) enhance the AAV vector-mediated transgene expression and the killing effect by AAVtk/GCV system. The enhancement of transgene expression was observed in a concentration-dependent manner on human laryngeal carcinoma cells (HEp-2 cells) and HeLa cells. Southern analysis confirmed that etoposide enhanced the double-strand synthesis of the AAV vector genome in HEp-2 cells and HeLa cells. The cells were efficiently killed by AAVtk/GCV system, as expected. More importantly, both etoposide and camptothecin augmented the cytocidal effect of the AAVtk/GCV system. These findings suggest that the combination of AAV-mediated suicide gene therapy and treatment with topoisomerase inhibitors may have synergistic therapeutic effects in the treatment of cancers.

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Copy and paste a formatted citation
Spandidos Publications style
Kanazawa T, Mizukami H, Nishino H, Okada T, Hanazono Y, Kume A, Kitamura K, Ichimura K and Ozawa K: Topoisomerase inhibitors enhance the cytocidal effect of AAV-HSVtk/ganciclovir on head and neck cancer cells. Int J Oncol 25: 729-735, 2004.
APA
Kanazawa, T., Mizukami, H., Nishino, H., Okada, T., Hanazono, Y., Kume, A. ... Ozawa, K. (2004). Topoisomerase inhibitors enhance the cytocidal effect of AAV-HSVtk/ganciclovir on head and neck cancer cells. International Journal of Oncology, 25, 729-735. https://doi.org/10.3892/ijo.25.3.729
MLA
Kanazawa, T., Mizukami, H., Nishino, H., Okada, T., Hanazono, Y., Kume, A., Kitamura, K., Ichimura, K., Ozawa, K."Topoisomerase inhibitors enhance the cytocidal effect of AAV-HSVtk/ganciclovir on head and neck cancer cells". International Journal of Oncology 25.3 (2004): 729-735.
Chicago
Kanazawa, T., Mizukami, H., Nishino, H., Okada, T., Hanazono, Y., Kume, A., Kitamura, K., Ichimura, K., Ozawa, K."Topoisomerase inhibitors enhance the cytocidal effect of AAV-HSVtk/ganciclovir on head and neck cancer cells". International Journal of Oncology 25, no. 3 (2004): 729-735. https://doi.org/10.3892/ijo.25.3.729
Copy and paste a formatted citation
x
Spandidos Publications style
Kanazawa T, Mizukami H, Nishino H, Okada T, Hanazono Y, Kume A, Kitamura K, Ichimura K and Ozawa K: Topoisomerase inhibitors enhance the cytocidal effect of AAV-HSVtk/ganciclovir on head and neck cancer cells. Int J Oncol 25: 729-735, 2004.
APA
Kanazawa, T., Mizukami, H., Nishino, H., Okada, T., Hanazono, Y., Kume, A. ... Ozawa, K. (2004). Topoisomerase inhibitors enhance the cytocidal effect of AAV-HSVtk/ganciclovir on head and neck cancer cells. International Journal of Oncology, 25, 729-735. https://doi.org/10.3892/ijo.25.3.729
MLA
Kanazawa, T., Mizukami, H., Nishino, H., Okada, T., Hanazono, Y., Kume, A., Kitamura, K., Ichimura, K., Ozawa, K."Topoisomerase inhibitors enhance the cytocidal effect of AAV-HSVtk/ganciclovir on head and neck cancer cells". International Journal of Oncology 25.3 (2004): 729-735.
Chicago
Kanazawa, T., Mizukami, H., Nishino, H., Okada, T., Hanazono, Y., Kume, A., Kitamura, K., Ichimura, K., Ozawa, K."Topoisomerase inhibitors enhance the cytocidal effect of AAV-HSVtk/ganciclovir on head and neck cancer cells". International Journal of Oncology 25, no. 3 (2004): 729-735. https://doi.org/10.3892/ijo.25.3.729
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