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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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October 2004 Volume 25 Issue 4

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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October 2004 Volume 25 Issue 4

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Article

Transcription factor NF-Y regulates mdr1 expression through binding to inverted CCAAT sequence in drug-resistant human squamous carcinoma cells.

  • Authors:
    • Hirohiko Okamura
    • Kaya Yoshida
    • Eiko Sasaki
    • Hiroyuki Morimoto
    • Tatsuji Haneji

  • View Affiliations / Copyright

    Affiliations: Department of Histology and Oral Histology, School of Dentistry, The University of Tokushima, 18-15, 3 Kuramoto-cho, Tokushima 770-8504, Japan. okamura@dent.tokushima-u.ac.jp
  • Pages: 1031-1038
    |
    Published online on: October 1, 2004
       https://doi.org/10.3892/ijo.25.4.1031
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Abstract

In this study, the expression and transcriptional regulation of the multidrug resistance-1 (MDR1) gene in multidrug-resistant SCCTF cells and -sensitive SCCKN cells derived from human squamous carcinoma were investigated. RT-PCR revealed that mdr1 mRNA was highly expressed in SCCTF cells while it was under the limit of detection in SCCKN cells. With an electrophoretic mobility shift assay using the mdr1 promoter region, a DNA-protein complex was detected strongly in SCCTF cells, but weakly in SCCKN cells. Incubation of the DNA-protein complex with an anti-NF-Y antibody caused a supershift in the migration to a position near the origin of the gel. Chromatin immunoprecipitation assay with an anti-NF-Y antibody showed that NF-Y binds to mdr1 promoter in SCCTF cells. The mdr1 promoter region including its NF-Y binding sequence was cloned into the luciferase reporter plasmid pGL3-basic vector, and this vector was used to transfect SCCTF and SCCKN cells. The luciferase assay showed that the inverted CCAAT sequence in the mdr1 promoter region is involved in the positive regulation of mdr1 promoter activity. NF-YA protein was expressed at higher levels in SCCTF cells than that in SCCKN cells. Hoechst dye staining also showed that MDR1 protein acts more effectively as an efflux pump in SCCTF cells than that in SCCKN cells.

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Copy and paste a formatted citation
Spandidos Publications style
Okamura H, Yoshida K, Sasaki E, Morimoto H and Haneji T: Transcription factor NF-Y regulates mdr1 expression through binding to inverted CCAAT sequence in drug-resistant human squamous carcinoma cells.. Int J Oncol 25: 1031-1038, 2004.
APA
Okamura, H., Yoshida, K., Sasaki, E., Morimoto, H., & Haneji, T. (2004). Transcription factor NF-Y regulates mdr1 expression through binding to inverted CCAAT sequence in drug-resistant human squamous carcinoma cells.. International Journal of Oncology, 25, 1031-1038. https://doi.org/10.3892/ijo.25.4.1031
MLA
Okamura, H., Yoshida, K., Sasaki, E., Morimoto, H., Haneji, T."Transcription factor NF-Y regulates mdr1 expression through binding to inverted CCAAT sequence in drug-resistant human squamous carcinoma cells.". International Journal of Oncology 25.4 (2004): 1031-1038.
Chicago
Okamura, H., Yoshida, K., Sasaki, E., Morimoto, H., Haneji, T."Transcription factor NF-Y regulates mdr1 expression through binding to inverted CCAAT sequence in drug-resistant human squamous carcinoma cells.". International Journal of Oncology 25, no. 4 (2004): 1031-1038. https://doi.org/10.3892/ijo.25.4.1031
Copy and paste a formatted citation
x
Spandidos Publications style
Okamura H, Yoshida K, Sasaki E, Morimoto H and Haneji T: Transcription factor NF-Y regulates mdr1 expression through binding to inverted CCAAT sequence in drug-resistant human squamous carcinoma cells.. Int J Oncol 25: 1031-1038, 2004.
APA
Okamura, H., Yoshida, K., Sasaki, E., Morimoto, H., & Haneji, T. (2004). Transcription factor NF-Y regulates mdr1 expression through binding to inverted CCAAT sequence in drug-resistant human squamous carcinoma cells.. International Journal of Oncology, 25, 1031-1038. https://doi.org/10.3892/ijo.25.4.1031
MLA
Okamura, H., Yoshida, K., Sasaki, E., Morimoto, H., Haneji, T."Transcription factor NF-Y regulates mdr1 expression through binding to inverted CCAAT sequence in drug-resistant human squamous carcinoma cells.". International Journal of Oncology 25.4 (2004): 1031-1038.
Chicago
Okamura, H., Yoshida, K., Sasaki, E., Morimoto, H., Haneji, T."Transcription factor NF-Y regulates mdr1 expression through binding to inverted CCAAT sequence in drug-resistant human squamous carcinoma cells.". International Journal of Oncology 25, no. 4 (2004): 1031-1038. https://doi.org/10.3892/ijo.25.4.1031
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