Ubiquilin-1 is a novel HASH-1-complexing protein that regulates levels of neuronal bHLH transcription factors in human neuroblastoma cells

  • Authors:
    • Paula Persson
    • Marie-Thérése Stockhausen
    • Sven Påhlman
    • Håkan Axelson
  • View Affiliations

  • Published online on: Monday, November 1, 2004
  • Pages: 1213-1221 DOI: 10.3892/ijo.25.5.1213

Abstract

The basic helix-loop-helix (bHLH) transcription factor mammalian achaete-scute homologue-1 (MASH-1 in mouse and HASH-1 in humans) is expressed in specific subsets of embryonic neuronal precursors of both the peripheral and central nervous systems. This gene is essential for development of olfactory and most peripheral autonomic neurons. Neuro-blastoma is a pediatric malignancy derived from sympathetic nervous system precursors and HASH-1 is expressed in a majority of neuroblastoma tumors and cell lines, indicating the immature phenotype of these cells. Using a human neuroblastoma cDNA library and the yeast two-hybrid system to identify novel HASH-1-interacting proteins, we isolated ubiquilin-1 (DA41, hPLIC-1), a gene that contains multiple ubiquitin-related domains. Further analyses showed that ubiquilin-1 interacts not only with HASH-1, but also with other tissue-specific bHLH proteins, including HES-1. Overexpression of ubiquilin-1 led to accumulation of HASH-1 and HES-1. Moreover, ubiquilin-1 was translocated from the cytoplasm to the nucleus upon co-expression with HASH-1. These results indicate that ubiquilin-1 plays an active role in the precise regulation of HASH-1 and of other tissue-specific bHLH proteins.

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November 2004
Volume 25 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

2015 Impact Factor: 3.018
Ranked #30/211 Oncology
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APA
Persson, P., Stockhausen, M., Påhlman, S., & Axelson, H. (2004). Ubiquilin-1 is a novel HASH-1-complexing protein that regulates levels of neuronal bHLH transcription factors in human neuroblastoma cells. International Journal of Oncology, 25, 1213-1221. http://dx.doi.org/10.3892/ijo.25.5.1213
MLA
Persson, P., Stockhausen, M., Påhlman, S., Axelson, H."Ubiquilin-1 is a novel HASH-1-complexing protein that regulates levels of neuronal bHLH transcription factors in human neuroblastoma cells". International Journal of Oncology 25.5 (2004): 1213-1221.
Chicago
Persson, P., Stockhausen, M., Påhlman, S., Axelson, H."Ubiquilin-1 is a novel HASH-1-complexing protein that regulates levels of neuronal bHLH transcription factors in human neuroblastoma cells". International Journal of Oncology 25, no. 5 (2004): 1213-1221. http://dx.doi.org/10.3892/ijo.25.5.1213