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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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November 2004 Volume 25 Issue 5

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Medicine International

An International Open Access Journal Devoted to General Medicine.

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November 2004 Volume 25 Issue 5

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Article

Allele-specific methylation analysis on upstream promoter region of H19 by methylation-specific PCR with confronting two-pair primers

  • Authors:
    • Hiromi Sasamoto
    • Takeshi Nagasaka
    • Kenji Notohara
    • Kazuhide Ozaki
    • Hiroshi Isozaki
    • Noriaki Tanaka
    • Nagahide Matsubara

  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterological Surgery and Surgical Oncology, Okayama University Graduate School of Medicine and Dentistry, Okayama 700-8558, Japan
  • Pages: 1273-1278
    |
    Published online on: November 1, 2004
       https://doi.org/10.3892/ijo.25.5.1273
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Abstract

H19 and IGF2 genes are imprinted genes and expressed differently depending on whether they are carried by a chromosome of maternal or paternal origin; H19 is expressed only from the maternal allele and IGF2 only from the paternally inherited allele. The upstream promoter region of H19 has the imprinting-control region (ICR) or CTCF binding sites, where the methylation status of this region is critical to the regulation of imprinting of the H19/IGF2 locus located in chromosome 11p15. There are various reports on imprinting disorders in this region. In colorectal cancer aberrant biallelic methylation of CTCF binding site has been reported, and aberrant hypomethylation of this region in bladder cancer. Thus, certain human neoplasms have either hyper- or hypo-methylation in the ICR. Hence it is still difficult to analyze allele-specific methylation disorder of the region, or differentially methylated regions (DMR), locate upstream of H19. Here we report a new method, which could distinguish paternal epigenetic or maternal epigenetic pattern by a single PCR assay, to combine methylation-specific PCR and PCR with confronting two-pair primers (MSP-CTPP). Using this method, we investigated the region close to H19 ICR in 161 colorectal cancer and 65 gastric cancer cases.

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Copy and paste a formatted citation
Spandidos Publications style
Sasamoto H, Nagasaka T, Notohara K, Ozaki K, Isozaki H, Tanaka N and Matsubara N: Allele-specific methylation analysis on upstream promoter region of H19 by methylation-specific PCR with confronting two-pair primers. Int J Oncol 25: 1273-1278, 2004.
APA
Sasamoto, H., Nagasaka, T., Notohara, K., Ozaki, K., Isozaki, H., Tanaka, N., & Matsubara, N. (2004). Allele-specific methylation analysis on upstream promoter region of H19 by methylation-specific PCR with confronting two-pair primers. International Journal of Oncology, 25, 1273-1278. https://doi.org/10.3892/ijo.25.5.1273
MLA
Sasamoto, H., Nagasaka, T., Notohara, K., Ozaki, K., Isozaki, H., Tanaka, N., Matsubara, N."Allele-specific methylation analysis on upstream promoter region of H19 by methylation-specific PCR with confronting two-pair primers". International Journal of Oncology 25.5 (2004): 1273-1278.
Chicago
Sasamoto, H., Nagasaka, T., Notohara, K., Ozaki, K., Isozaki, H., Tanaka, N., Matsubara, N."Allele-specific methylation analysis on upstream promoter region of H19 by methylation-specific PCR with confronting two-pair primers". International Journal of Oncology 25, no. 5 (2004): 1273-1278. https://doi.org/10.3892/ijo.25.5.1273
Copy and paste a formatted citation
x
Spandidos Publications style
Sasamoto H, Nagasaka T, Notohara K, Ozaki K, Isozaki H, Tanaka N and Matsubara N: Allele-specific methylation analysis on upstream promoter region of H19 by methylation-specific PCR with confronting two-pair primers. Int J Oncol 25: 1273-1278, 2004.
APA
Sasamoto, H., Nagasaka, T., Notohara, K., Ozaki, K., Isozaki, H., Tanaka, N., & Matsubara, N. (2004). Allele-specific methylation analysis on upstream promoter region of H19 by methylation-specific PCR with confronting two-pair primers. International Journal of Oncology, 25, 1273-1278. https://doi.org/10.3892/ijo.25.5.1273
MLA
Sasamoto, H., Nagasaka, T., Notohara, K., Ozaki, K., Isozaki, H., Tanaka, N., Matsubara, N."Allele-specific methylation analysis on upstream promoter region of H19 by methylation-specific PCR with confronting two-pair primers". International Journal of Oncology 25.5 (2004): 1273-1278.
Chicago
Sasamoto, H., Nagasaka, T., Notohara, K., Ozaki, K., Isozaki, H., Tanaka, N., Matsubara, N."Allele-specific methylation analysis on upstream promoter region of H19 by methylation-specific PCR with confronting two-pair primers". International Journal of Oncology 25, no. 5 (2004): 1273-1278. https://doi.org/10.3892/ijo.25.5.1273
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