Analysis of isoform specific function of PP1 catalytic subunits in mammalian cells using siRNA
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- Published online on: November 1, 2004 https://doi.org/10.3892/ijo.25.5.1383
- Pages: 1383-1388
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Abstract
Protein phosphatase type 1 (PP1) is involved in the regulation of numerous cell functions in mammalian cells. The major isoforms of PP1 catalytic subunit (PP1C)α, γ1 and δ have nearly identical catalytic domain, but they vary in sequences at the amino and carboxyl termini. We previously showed that PP1Cα is highly expressed in rat hepatoma cells. To examine isoform specific function of PP1C, each isoform was depleted from HeLa cells by RNA interference. The PP1Cα-depleted cells rounded up and showed increased cell death, indicating that PP1Cα is essential in cell proliferation. PP1Cγ1-depleted cells slightly rounded up and have decreased G1 phase population and increased S phase population. The PP1Cδ-depleted cells were enlarged, and appeared flat and rich in lamellipodia. These data suggested that each PP1C isoform has non-redundant function in vivo.