Transactivation of E-cadherin is not involved in the activity of EGF receptor in colorectal carcinoma cells

  • Authors:
    • Vlasta Sovová
    • Dana Kucerová
    • Martina Vojtechová
    • Eva Sloncová
    • Zdena Tuhácková
  • View Affiliations

  • Published online on: November 1, 2004     https://doi.org/10.3892/ijo.25.5.1459
  • Pages: 1459-1464
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Abstract

In epithelial cells, the cell surface glycoprotein E-cadherin is a key molecule in the establishment of cell-cell adhesion. In addition to its contribution to cell adhesion, E-cadherin was found to induce ligand-independent activation of the EGF receptor (EGFR), likely as a result of their co-clustering. As it has also been reported that ligand activation of the overexpressed EGFRs disturb E-cadherin-mediated cell-cell adhesion, we analyzed E-cadherin-EGFR interactions and their consequences in A431 cells and in two colorectal cancer cell lines using immunoblotting and analyzes of several protein kinase activities. Activation of the PI3-K/Akt/GSK-3 signaling pathway upon EGF treatment that we observed in the analyzed cells indicates that EGFRs are functional even in the colorectal cancer cells containing a low density of EGFRs. The transactivation of EGFR by E-cadherin did not occur either in the colorectal cancer cells tested or in A431 cells containing a high density of both EGFRs and E-cadherin on their surface. This observation suggests that high amounts of both molecules on the surface of tumour cells did not predetermine ligand-independent activation of EGFRs.

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November 2004
Volume 25 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Sovová V, Kucerová D, Vojtechová M, Sloncová E and Tuhácková Z: Transactivation of E-cadherin is not involved in the activity of EGF receptor in colorectal carcinoma cells. Int J Oncol 25: 1459-1464, 2004
APA
Sovová, V., Kucerová, D., Vojtechová, M., Sloncová, E., & Tuhácková, Z. (2004). Transactivation of E-cadherin is not involved in the activity of EGF receptor in colorectal carcinoma cells. International Journal of Oncology, 25, 1459-1464. https://doi.org/10.3892/ijo.25.5.1459
MLA
Sovová, V., Kucerová, D., Vojtechová, M., Sloncová, E., Tuhácková, Z."Transactivation of E-cadherin is not involved in the activity of EGF receptor in colorectal carcinoma cells". International Journal of Oncology 25.5 (2004): 1459-1464.
Chicago
Sovová, V., Kucerová, D., Vojtechová, M., Sloncová, E., Tuhácková, Z."Transactivation of E-cadherin is not involved in the activity of EGF receptor in colorectal carcinoma cells". International Journal of Oncology 25, no. 5 (2004): 1459-1464. https://doi.org/10.3892/ijo.25.5.1459