The der(12)t(12;16) breakpoint in an acute leukaemia case targets a Sec7 domain containing protein

  • Authors:
    • Anniek Corveleyn
    • Iwona Wlodarska
    • Cristina Mecucci
    • Peter Marynen
  • View Affiliations

  • Published online on: April 1, 2005     https://doi.org/10.3892/ijo.26.4.1111
  • Pages: 1111-1120
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Abstract

A balanced translocation t(12;16)(p13;p13) in a patient with AML-M1 and bone marrow eosinophilia was previously analysed by FISH. The 16p13 breakpoint was shown to occur in the MYH11 gene, the fusion partner of CBFβ in leukaemia patients with an inv(16) or a t(16;16), whereas the 12p13 breakpoint was shown to be present in cosmid c4H9. We present the molecular analysis of c4H9, resulting in the identification of a novel gene, SLAG. At the N-terminus, SLAG contains a Sec7 domain also found in proteins of the cytohesin family. In contrast to the cytohesins, no pleckstrin homology domain is present in SLAG. database searches identified several homologues, suggesting that SLAG defines a new subfamily of Sec7 proteins, characterised by an N-terminal Sec7 domain and a new C-terminal pleckstrin homology-like domain. The FISH data led to the hypothesis that rearrangement of SLAG might be involved in the pathogenesis of AML through the generation of a new fusion gene with MYH11. However, the putative SLAG-MYH11 fusions showed only weak transforming properties in NIH3T3 cells in a focus formation assay.

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April 2005
Volume 26 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Corveleyn A, Wlodarska I, Mecucci C and Marynen P: The der(12)t(12;16) breakpoint in an acute leukaemia case targets a Sec7 domain containing protein. Int J Oncol 26: 1111-1120, 2005
APA
Corveleyn, A., Wlodarska, I., Mecucci, C., & Marynen, P. (2005). The der(12)t(12;16) breakpoint in an acute leukaemia case targets a Sec7 domain containing protein. International Journal of Oncology, 26, 1111-1120. https://doi.org/10.3892/ijo.26.4.1111
MLA
Corveleyn, A., Wlodarska, I., Mecucci, C., Marynen, P."The der(12)t(12;16) breakpoint in an acute leukaemia case targets a Sec7 domain containing protein". International Journal of Oncology 26.4 (2005): 1111-1120.
Chicago
Corveleyn, A., Wlodarska, I., Mecucci, C., Marynen, P."The der(12)t(12;16) breakpoint in an acute leukaemia case targets a Sec7 domain containing protein". International Journal of Oncology 26, no. 4 (2005): 1111-1120. https://doi.org/10.3892/ijo.26.4.1111