Altered expression of members of the IGF-axis in clear cell renal cell carcinoma

  • Authors:
    • Masayuki Takahashi
    • Veronica Papavero
    • Jason Yuhas
    • Eric Kort
    • Hiro-Omi Kanayama
    • Susumu Kagawa
    • Robert C. Baxter
    • Ximing J. Yang
    • Steven G. Gray
    • Bin Tean Teh
  • View Affiliations

  • Published online on: April 1, 2005     https://doi.org/10.3892/ijo.26.4.923
  • Pages: 923-931
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Renal cell carcinoma (RCC) is a heterogeneous disease and its biology is poorly understood. The commonest subtype identified is clear cell RCC. The insulin-like growth factor axis is intimately involved with many cellular roles including that of renal development. Dysregulation of this axis has frequently been demonstrated in cancer. In this study, we examine the expression of several IGF-axis components, including receptors, ligands, and binding proteins in clear cell renal cell carcinoma. A series of clear cell RCCs with matched normal kidney from the same individuals were obtained. Total RNA was extracted and expression levels of genes examined using RNase protection analysis. We confirm the dysregulation of the IGF-axis within clear cell renal cell carcinoma including the upregulation of IGFBP-3, which is further validated by immunohistochemical staining on a tissue array containing 50 RCC: positive staining in 29/30 clear cell; 1/10 papillary and 0/10 chromophobe. In addition, we demonstrate that the expression of the A isoform of the insulin receptor is significantly upregulated, while that of IGFBP-5 are significantly downregulated in this tumour subtype.

Related Articles

Journal Cover

April 2005
Volume 26 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Takahashi M, Papavero V, Yuhas J, Kort E, Kanayama H, Kagawa S, Baxter RC, Yang XJ, Gray SG, Teh BT, Teh BT, et al: Altered expression of members of the IGF-axis in clear cell renal cell carcinoma. Int J Oncol 26: 923-931, 2005
APA
Takahashi, M., Papavero, V., Yuhas, J., Kort, E., Kanayama, H., Kagawa, S. ... Teh, B.T. (2005). Altered expression of members of the IGF-axis in clear cell renal cell carcinoma. International Journal of Oncology, 26, 923-931. https://doi.org/10.3892/ijo.26.4.923
MLA
Takahashi, M., Papavero, V., Yuhas, J., Kort, E., Kanayama, H., Kagawa, S., Baxter, R. C., Yang, X. J., Gray, S. G., Teh, B. T."Altered expression of members of the IGF-axis in clear cell renal cell carcinoma". International Journal of Oncology 26.4 (2005): 923-931.
Chicago
Takahashi, M., Papavero, V., Yuhas, J., Kort, E., Kanayama, H., Kagawa, S., Baxter, R. C., Yang, X. J., Gray, S. G., Teh, B. T."Altered expression of members of the IGF-axis in clear cell renal cell carcinoma". International Journal of Oncology 26, no. 4 (2005): 923-931. https://doi.org/10.3892/ijo.26.4.923