Proteome-based identification of molecular markers predicting chemosensitivity to each category of anticancer agents in human gliomas

  • Authors:
    • Yasuo Iwadate
    • Tsukasa Sakaida
    • Takashi Saegusa
    • Takaki Hiwasa
    • Masaki Takiguchi
    • Shuichi Fujimoto
    • Akira Yamaura
  • View Affiliations

  • Published online on: April 1, 2005     https://doi.org/10.3892/ijo.26.4.993
  • Pages: 993-998
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

To identify the protein markers that are clinically useful for predicting efficacy of anticancer agents, we investigated the correlation between the proteome profiling patterns and the in vitro chemosensitivity in human gliomas. The proteome of 93 surgical samples were analyzed with two-dimensional gel electrophoresis (2DE) and mass spectrometry. The in vitro chemosensitivities to 10 different kinds of anticancer agents (cyclophosphamide, nimustine, cisplatin, cytosine arabinoside, mitomycin C, peplomycin, adriamycin, etoposide, vincristine, paclitaxel) were measured by flow cytometric detection of apoptosis. We identified a set of 41 proteins that significantly affected the in vitro chemosensitivity to each category of anticancer agents. Many of the proteins that correlated with chemoresistance were categorized into the signal transduction proteins including the G-proteins. The present study showed that the proteome analysis using 2DE could provide a list of proteins that may be the potential predictive markers for chemosensitivity in human gliomas. They can also be direct and rational targets for anti-glioma therapy and be used for sensitization to the conventional chemotherapeutic regimens.

Related Articles

Journal Cover

April 2005
Volume 26 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Iwadate Y, Sakaida T, Saegusa T, Hiwasa T, Takiguchi M, Fujimoto S and Yamaura A: Proteome-based identification of molecular markers predicting chemosensitivity to each category of anticancer agents in human gliomas. Int J Oncol 26: 993-998, 2005
APA
Iwadate, Y., Sakaida, T., Saegusa, T., Hiwasa, T., Takiguchi, M., Fujimoto, S., & Yamaura, A. (2005). Proteome-based identification of molecular markers predicting chemosensitivity to each category of anticancer agents in human gliomas. International Journal of Oncology, 26, 993-998. https://doi.org/10.3892/ijo.26.4.993
MLA
Iwadate, Y., Sakaida, T., Saegusa, T., Hiwasa, T., Takiguchi, M., Fujimoto, S., Yamaura, A."Proteome-based identification of molecular markers predicting chemosensitivity to each category of anticancer agents in human gliomas". International Journal of Oncology 26.4 (2005): 993-998.
Chicago
Iwadate, Y., Sakaida, T., Saegusa, T., Hiwasa, T., Takiguchi, M., Fujimoto, S., Yamaura, A."Proteome-based identification of molecular markers predicting chemosensitivity to each category of anticancer agents in human gliomas". International Journal of Oncology 26, no. 4 (2005): 993-998. https://doi.org/10.3892/ijo.26.4.993