Suppression of tumor growth in human gastric cancer with HER2 overexpression by an anti-HER2 antibody in a murine model

  • Authors:
    • Yoko Matsui
    • Masafumi Inomata
    • Manabu Tojigamori
    • Kazuya Sonoda
    • Norio Shiraishi
    • Seigo Kitano
  • View Affiliations

  • Published online on: September 1, 2005     https://doi.org/10.3892/ijo.27.3.681
  • Pages: 681-685
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Abstract

New modalities are necessary for the treatment of patients with unresectable gastric cancer. The aim of this study was to investigate whether or not anti-HER2 antibody could suppress the growth of human gastric cancer cells with HER2 overexpression in vitro and in vivo. Four human gastric cancer cell lines, NCI-N87, MKN-45P, Kato-III, and MKN-1, were used in this study. The suppression of cell proliferation in vitro and of subcutaneous tumor growth in a nude mouse model after treatment with trastuzumab was examined. The expression of HER2 protein was investigated by Western blot analysis. The effect of trastuzumab on the survival rate of nude mice with peritoneal dissemination was examined. Trastuzumab significantly reduced proliferative activity in NCI-N87, a HER2-overexpressing human gastric cancer cell line, in vitro. In the nude mouse model with transplanted subcutaneous tumor, trastuzumab significantly suppressed the tumor growth of NCI-N87 cells, and then HER2 expression was reduced. Trastuzumab improved the survival rate of mice with peritoneal dissemination of MKN-45P cells. Trastuzumab therapy is a potential candidate for a novel treatment of HER2-overexpressing gastric cancer.

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September 2005
Volume 27 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Matsui Y, Inomata M, Tojigamori M, Sonoda K, Shiraishi N and Kitano S: Suppression of tumor growth in human gastric cancer with HER2 overexpression by an anti-HER2 antibody in a murine model. Int J Oncol 27: 681-685, 2005
APA
Matsui, Y., Inomata, M., Tojigamori, M., Sonoda, K., Shiraishi, N., & Kitano, S. (2005). Suppression of tumor growth in human gastric cancer with HER2 overexpression by an anti-HER2 antibody in a murine model. International Journal of Oncology, 27, 681-685. https://doi.org/10.3892/ijo.27.3.681
MLA
Matsui, Y., Inomata, M., Tojigamori, M., Sonoda, K., Shiraishi, N., Kitano, S."Suppression of tumor growth in human gastric cancer with HER2 overexpression by an anti-HER2 antibody in a murine model". International Journal of Oncology 27.3 (2005): 681-685.
Chicago
Matsui, Y., Inomata, M., Tojigamori, M., Sonoda, K., Shiraishi, N., Kitano, S."Suppression of tumor growth in human gastric cancer with HER2 overexpression by an anti-HER2 antibody in a murine model". International Journal of Oncology 27, no. 3 (2005): 681-685. https://doi.org/10.3892/ijo.27.3.681