ephrin-B2 overexpression enhances integrin-mediated ECM-attachment and migration of B16 melanoma cells

  • Authors:
    • Stefanie Meyer
    • Christian Hafner
    • Markus Guba
    • Stefanie Flegel
    • Edward K. Geissler
    • Bernd Becker
    • Gudrun E. Koehl
    • Evelyn Orsó
    • Michael Landthaler
    • Thomas Vogt
  • View Affiliations

  • Published online on: November 1, 2005     https://doi.org/10.3892/ijo.27.5.1197
  • Pages: 1197-1206
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Abstract

Eph-receptor tyrosine kinases (Eph-RTKs) and their membrane-bound receptor-like ligands, the ephrins, represent a cell-cell signaling system that directs cellular migration during development. Differential expression in cancer suggests similar roles in tumor progression. We have previously shown that ephrin-B2 mRNA is overexpressed in advanced malignant melanomas (MM). In this study, immunohistochemistry revealed a most prominent expression of ephrin-B2 in the invasive front of advanced MM. Therefore, we addressed the question of whether ephrin-B2 signaling modulates MM cell migration and matrix interaction. Using a wild-type ephrin-B2-negative B16 mouse MM subclone we show that overexpression of ephrin-B2 leads to the formation of multiple lamellipodia, enhanced polymerisation of actin fibers, and induction of focal adhesion complexes with constitutive activation of focal adhesion kinase. Consequently, ephrin-B2-overexpressing B16 cells display a significant increase of β1-integrin-mediated attachment to matrix components, preferentially laminin and fibronectin. As a further effect of ephrin-B2 overexpression, we observed an accelerated migration in both Boyden chamber invasion experiments as well as in in vitro scratch-wound assays. We conclude that ephrin-B2 can act as a major modulator of cell migration and matrix interactions of MM cells, which possibly contributes to the expansion and metastatic spread of MM in vivo.

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November 2005
Volume 27 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Meyer S, Hafner C, Guba M, Flegel S, Geissler EK, Becker B, Koehl GE, Orsó E, Landthaler M, Vogt T, Vogt T, et al: ephrin-B2 overexpression enhances integrin-mediated ECM-attachment and migration of B16 melanoma cells. Int J Oncol 27: 1197-1206, 2005.
APA
Meyer, S., Hafner, C., Guba, M., Flegel, S., Geissler, E.K., Becker, B. ... Vogt, T. (2005). ephrin-B2 overexpression enhances integrin-mediated ECM-attachment and migration of B16 melanoma cells. International Journal of Oncology, 27, 1197-1206. https://doi.org/10.3892/ijo.27.5.1197
MLA
Meyer, S., Hafner, C., Guba, M., Flegel, S., Geissler, E. K., Becker, B., Koehl, G. E., Orsó, E., Landthaler, M., Vogt, T."ephrin-B2 overexpression enhances integrin-mediated ECM-attachment and migration of B16 melanoma cells". International Journal of Oncology 27.5 (2005): 1197-1206.
Chicago
Meyer, S., Hafner, C., Guba, M., Flegel, S., Geissler, E. K., Becker, B., Koehl, G. E., Orsó, E., Landthaler, M., Vogt, T."ephrin-B2 overexpression enhances integrin-mediated ECM-attachment and migration of B16 melanoma cells". International Journal of Oncology 27, no. 5 (2005): 1197-1206. https://doi.org/10.3892/ijo.27.5.1197