IGFBP-3 expression in hepatocellular carcinoma involves abnormalities in TGF-β and/or Rb signaling pathways

  • Authors:
    • Eiichiro Yumoto
    • Harushige Nakatsukasa
    • Tadashi Hanafusa
    • Yasuhiro Yumoto
    • Kazuhiro Nouso
    • Eiji Matsumoto
    • Toru Onishi
    • Yoshitaka Takuma
    • Hironori Tanaka
    • Tatsuya Fujikawa
    • Mayumi Suzuki
    • Masayuki Uemura
    • Yasushi Shiratori
  • View Affiliations

  • Published online on: November 1, 2005     https://doi.org/10.3892/ijo.27.5.1223
  • Pages: 1223-1230
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Abstract

Insulin-like growth factor binding protein-3 (IGFBP-3) is a mediator of growth suppression signals and a putative tumor suppressor gene. The growth suppression mechanisms of IGFBP-3 have not been well clarified. We examined the expression of IGFBP-3 transcripts in human hepatocellular carcinoma (HCC) and the relationship between IGFBP-3 expression and the transforming growth factor-β (TGF-β) and/or retinoblastoma (Rb) signaling pathways. In situ hybridization revealed IGFBP-3 transcripts in cancer cells in 6 of 57 (10%) HCCs, including moderately and poorly differentiated HCCs with intrahepatic metastasis. In contrast, all lung metastatic nodules of 4 HCCs showed IGFBP-3 transcripts in cancer cells. The cDNA microarray showed that genes for the TGF-β pathway and Rb were up-regulated in IGFBP-3-expressing HCCs. In 6 HCCs presenting IGFBP-3, immunohistochemical analyses showed abnormalities in the TGF-β and/or Rb pathways; the loss of phosphorylated-Smad2 was observed in 2, and overexpression of phosphorylated-Rb was observed in the remaining 4 HCCs. The present study suggests that IGFBP-3 mediates growth suppression signals via the TGF-β and/or Rb pathways in HCC.

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November 2005
Volume 27 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Yumoto E, Nakatsukasa H, Hanafusa T, Yumoto Y, Nouso K, Matsumoto E, Onishi T, Takuma Y, Tanaka H, Fujikawa T, Fujikawa T, et al: IGFBP-3 expression in hepatocellular carcinoma involves abnormalities in TGF-β and/or Rb signaling pathways. Int J Oncol 27: 1223-1230, 2005
APA
Yumoto, E., Nakatsukasa, H., Hanafusa, T., Yumoto, Y., Nouso, K., Matsumoto, E. ... Shiratori, Y. (2005). IGFBP-3 expression in hepatocellular carcinoma involves abnormalities in TGF-β and/or Rb signaling pathways. International Journal of Oncology, 27, 1223-1230. https://doi.org/10.3892/ijo.27.5.1223
MLA
Yumoto, E., Nakatsukasa, H., Hanafusa, T., Yumoto, Y., Nouso, K., Matsumoto, E., Onishi, T., Takuma, Y., Tanaka, H., Fujikawa, T., Suzuki, M., Uemura, M., Shiratori, Y."IGFBP-3 expression in hepatocellular carcinoma involves abnormalities in TGF-β and/or Rb signaling pathways". International Journal of Oncology 27.5 (2005): 1223-1230.
Chicago
Yumoto, E., Nakatsukasa, H., Hanafusa, T., Yumoto, Y., Nouso, K., Matsumoto, E., Onishi, T., Takuma, Y., Tanaka, H., Fujikawa, T., Suzuki, M., Uemura, M., Shiratori, Y."IGFBP-3 expression in hepatocellular carcinoma involves abnormalities in TGF-β and/or Rb signaling pathways". International Journal of Oncology 27, no. 5 (2005): 1223-1230. https://doi.org/10.3892/ijo.27.5.1223