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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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December 2005 Volume 27 Issue 6

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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December 2005 Volume 27 Issue 6

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Article

Evaluation of p16 and Id1 status and endogenous reference genes in human chondrosarcoma by real-time PCR

  • Authors:
    • Julia Asp
    • Camilla Brantsing
    • Karina Lövstedt
    • Maria Serena Benassi
    • Sven Inerot
    • Gabriella Gamberi
    • Piero Picci
    • Anders Lindahl

  • View Affiliations / Copyright

    Affiliations: Research Centre for Endocrinology and Metabolism, Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, Göteborg University, Göteborg, Sweden
  • Pages: 1577-1582
    |
    Published online on: December 1, 2005
       https://doi.org/10.3892/ijo.27.6.1577
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Abstract

Both the tumour suppressor, p16, and the helix-loop-helix transcription factor, Id1, have been assigned roles in tumour growth in general and appear to be involved in chondrosarcoma. Id1 has further been found to repress the expression of p16. Therefore, the mRNA expression of these two genes was studied by real-time PCR in a search for prognostic markers in human chondrosarcoma. To get reliable quantitative data, however, the choice of endogenous reference gene for use in the assay is important. Therefore, eleven different endogenous reference genes were evaluated in chondrosarcoma cells and articular chondrocytes. 18S rRNA appeared to be the best choice to use as endogenous reference gene, since it was suitable for both kinds of cells. Several of the other reference genes tested showed variation between individuals or between normal chondrocytes and chondrosarcoma cells. This demonstrates the importance of using a correct endogenous reference gene to get reliable results from quantitative measurements. Both p16 and Id1 showed varied gene expression patterns among the samples and none of these genes could be significantly correlated to prognosis.

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Copy and paste a formatted citation
Spandidos Publications style
Asp J, Brantsing C, Lövstedt K, Benassi MS, Inerot S, Gamberi G, Picci P and Lindahl A: Evaluation of p16 and Id1 status and endogenous reference genes in human chondrosarcoma by real-time PCR. Int J Oncol 27: 1577-1582, 2005.
APA
Asp, J., Brantsing, C., Lövstedt, K., Benassi, M.S., Inerot, S., Gamberi, G. ... Lindahl, A. (2005). Evaluation of p16 and Id1 status and endogenous reference genes in human chondrosarcoma by real-time PCR. International Journal of Oncology, 27, 1577-1582. https://doi.org/10.3892/ijo.27.6.1577
MLA
Asp, J., Brantsing, C., Lövstedt, K., Benassi, M. S., Inerot, S., Gamberi, G., Picci, P., Lindahl, A."Evaluation of p16 and Id1 status and endogenous reference genes in human chondrosarcoma by real-time PCR". International Journal of Oncology 27.6 (2005): 1577-1582.
Chicago
Asp, J., Brantsing, C., Lövstedt, K., Benassi, M. S., Inerot, S., Gamberi, G., Picci, P., Lindahl, A."Evaluation of p16 and Id1 status and endogenous reference genes in human chondrosarcoma by real-time PCR". International Journal of Oncology 27, no. 6 (2005): 1577-1582. https://doi.org/10.3892/ijo.27.6.1577
Copy and paste a formatted citation
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Spandidos Publications style
Asp J, Brantsing C, Lövstedt K, Benassi MS, Inerot S, Gamberi G, Picci P and Lindahl A: Evaluation of p16 and Id1 status and endogenous reference genes in human chondrosarcoma by real-time PCR. Int J Oncol 27: 1577-1582, 2005.
APA
Asp, J., Brantsing, C., Lövstedt, K., Benassi, M.S., Inerot, S., Gamberi, G. ... Lindahl, A. (2005). Evaluation of p16 and Id1 status and endogenous reference genes in human chondrosarcoma by real-time PCR. International Journal of Oncology, 27, 1577-1582. https://doi.org/10.3892/ijo.27.6.1577
MLA
Asp, J., Brantsing, C., Lövstedt, K., Benassi, M. S., Inerot, S., Gamberi, G., Picci, P., Lindahl, A."Evaluation of p16 and Id1 status and endogenous reference genes in human chondrosarcoma by real-time PCR". International Journal of Oncology 27.6 (2005): 1577-1582.
Chicago
Asp, J., Brantsing, C., Lövstedt, K., Benassi, M. S., Inerot, S., Gamberi, G., Picci, P., Lindahl, A."Evaluation of p16 and Id1 status and endogenous reference genes in human chondrosarcoma by real-time PCR". International Journal of Oncology 27, no. 6 (2005): 1577-1582. https://doi.org/10.3892/ijo.27.6.1577
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