CDK5 is a novel regulatory protein in PPARγ ligand-induced antiproliferation

  • Authors:
    • Eugene Kim
    • Fei Chen
    • Chia-Chi Wang
    • Lawrence E. Harrison
  • View Affiliations

  • Published online on: January 1, 2006     https://doi.org/10.3892/ijo.28.1.191
  • Pages: 191-194
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Abstract

Cyclin-dependent kinase 5 (Cdk5) is a member of the cyclin-dependent kinase family and has been studied mainly in the differentiation of post-mitotic neurons. The purpose of this study was to determine the presence of cdk5 expression and activity in colon cancer cells and to investigate its role in the regulation of PPARγ ligand-induced antiproliferation. We observed that cdk5 protein levels and kinase activity were elevated in both HT-29 cells and human tumor tissue in comparison to decreased levels in normal colonic mucosa. To elucidate cdk5's role in PPARγ ligand-induced antiproliferation of colon cancer cells, HT-29 cells were treated with ciglitazone. A dose- and time-dependent decrease in cell proliferation were observed after ciglitazone exposure, which correlated with a decrease in cdk5 protein expression and kinase activity. Importantly, these ciglitazone-induced antiproliferative changes were reversed when cdk5 was overexpressed. Although present, p35, the regulatory protein of cdk5, showed no significant changes in protein expression with the introduction of ciglitazone. This is the first report of cdk5/p35 expression and kinase activity in colon cancer cells, which is associated with ciglitazone-induced antiproliferation in HT-29 cells.

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January 2006
Volume 28 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Copy and paste a formatted citation
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Spandidos Publications style
Kim E, Chen F, Wang C and Harrison LE: CDK5 is a novel regulatory protein in PPARγ ligand-induced antiproliferation. Int J Oncol 28: 191-194, 2006.
APA
Kim, E., Chen, F., Wang, C., & Harrison, L.E. (2006). CDK5 is a novel regulatory protein in PPARγ ligand-induced antiproliferation. International Journal of Oncology, 28, 191-194. https://doi.org/10.3892/ijo.28.1.191
MLA
Kim, E., Chen, F., Wang, C., Harrison, L. E."CDK5 is a novel regulatory protein in PPARγ ligand-induced antiproliferation". International Journal of Oncology 28.1 (2006): 191-194.
Chicago
Kim, E., Chen, F., Wang, C., Harrison, L. E."CDK5 is a novel regulatory protein in PPARγ ligand-induced antiproliferation". International Journal of Oncology 28, no. 1 (2006): 191-194. https://doi.org/10.3892/ijo.28.1.191