Aberrant expression of histone deacetylase 6 in oral squamous cell carcinoma

  • Authors:
    • Takumi Sakuma
    • Katsuhiro Uzawa
    • Takeshi Onda
    • Masashi Shiiba
    • Hidetaka Yokoe
    • Takahiko Shibahara
    • Hideki Tanzawa
  • View Affiliations

  • Published online on: July 1, 2006     https://doi.org/10.3892/ijo.29.1.117
  • Pages: 117-124
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Abstract

The structure and function of chromatin can be altered by modifications to histone. Histone acetylation is a reversible process governed by histone acetyltransferases and histone deacetylases (HDACs). HDAC6 is a subtype of the HDAC family that deacetylates α-tubulin and increases cell motility. We investigated the expression levels of HDAC6 mRNA and protein expression in oral squamous cell carcinoma (OSCC)-derived cell lines and human primary OSCCs to elucidate the potential involvement of HDAC6 in OSCC. Using quantitative real-time reverse transcription polymerase chain reaction and Western blots on nine OSCC-derived cell lines and normal oral keratinocytes (NOKs), HDAC6 mRNA and protein expression were commonly up-regulated in all cell lines compared with the NOKs. Immunofluorescence analysis detected HDAC6 protein in the cytoplasm of OSCC cell lines. Similar to OSCC cell lines, high frequencies of HDAC6 up-regulation were evident in both mRNA (74%) and protein (51%) levels of primary tumors. Among the clinical variables analyzed, the clinical tumor stage was found to be associated with the HDAC6 expression states. The analysis indicated a significant difference in the HDAC6 expression level between the early stage (stage I and II) and advanced-stage (stage III and IV) tumors (P=0.014). These results suggest that HDAC6 expression may be correlated with tumor aggressiveness and offer clues to the planning of new treatments.

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July 2006
Volume 29 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Sakuma T, Uzawa K, Onda T, Shiiba M, Yokoe H, Shibahara T and Tanzawa H: Aberrant expression of histone deacetylase 6 in oral squamous cell carcinoma. Int J Oncol 29: 117-124, 2006
APA
Sakuma, T., Uzawa, K., Onda, T., Shiiba, M., Yokoe, H., Shibahara, T., & Tanzawa, H. (2006). Aberrant expression of histone deacetylase 6 in oral squamous cell carcinoma. International Journal of Oncology, 29, 117-124. https://doi.org/10.3892/ijo.29.1.117
MLA
Sakuma, T., Uzawa, K., Onda, T., Shiiba, M., Yokoe, H., Shibahara, T., Tanzawa, H."Aberrant expression of histone deacetylase 6 in oral squamous cell carcinoma". International Journal of Oncology 29.1 (2006): 117-124.
Chicago
Sakuma, T., Uzawa, K., Onda, T., Shiiba, M., Yokoe, H., Shibahara, T., Tanzawa, H."Aberrant expression of histone deacetylase 6 in oral squamous cell carcinoma". International Journal of Oncology 29, no. 1 (2006): 117-124. https://doi.org/10.3892/ijo.29.1.117