Differential regulation of p73 variants in response to cisplatin treatment in SH-SY5Y neuroblastoma cells

  • Authors:
    • Karine Million
    • Émilie Horvilleur
    • David Goldschneider
    • Malik Agina
    • Gilda Raguénez
    • Frédéric Tournier
    • Jean Bénard
    • Sétha Douc-Rasy
  • View Affiliations

  • Published online on: July 1, 2006     https://doi.org/10.3892/ijo.29.1.147
  • Pages: 147-154
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Abstract

The present study aims to investigate the role of p73 in response to cisplatin treatment in p53 wild-type neuroblastoma SH-SY5Y cells. Results showed that cisplatin induced a dose-dependent up-regulation of p53, p73, and a number of p53-responsive genes. Interestingly, endogenous Δexon2p73-expression was down-regulated by cisplatin treatment. Neither p21 nor GADD45 induction was observed in p53-deficient Lan-1 cells, although endogenous TAp73 expression was markedly induced. In the presence of cisplatin, exogenous TAp73 overexpression in SH-SY5Y cells induced p21 up-regulation without altering the apoptotic sub-G1 cell population. Moreover, siRNA-mediated suppression of TAp73 expression did not alter the sub-G1 population. Collectively, our results suggest that wt-p53 SH-SY5Y cells respond to cisplatin by inducing p73 isoform regulation and sustaining p53-dependent apoptosis that is independent of TAp73α.

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July 2006
Volume 29 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Million K, Horvilleur É, Goldschneider D, Agina M, Raguénez G, Tournier F, Bénard J and Douc-Rasy S: Differential regulation of p73 variants in response to cisplatin treatment in SH-SY5Y neuroblastoma cells. Int J Oncol 29: 147-154, 2006
APA
Million, K., Horvilleur, É., Goldschneider, D., Agina, M., Raguénez, G., Tournier, F. ... Douc-Rasy, S. (2006). Differential regulation of p73 variants in response to cisplatin treatment in SH-SY5Y neuroblastoma cells. International Journal of Oncology, 29, 147-154. https://doi.org/10.3892/ijo.29.1.147
MLA
Million, K., Horvilleur, É., Goldschneider, D., Agina, M., Raguénez, G., Tournier, F., Bénard, J., Douc-Rasy, S."Differential regulation of p73 variants in response to cisplatin treatment in SH-SY5Y neuroblastoma cells". International Journal of Oncology 29.1 (2006): 147-154.
Chicago
Million, K., Horvilleur, É., Goldschneider, D., Agina, M., Raguénez, G., Tournier, F., Bénard, J., Douc-Rasy, S."Differential regulation of p73 variants in response to cisplatin treatment in SH-SY5Y neuroblastoma cells". International Journal of Oncology 29, no. 1 (2006): 147-154. https://doi.org/10.3892/ijo.29.1.147