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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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September 2006 Volume 29 Issue 3

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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September 2006 Volume 29 Issue 3

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Article

CoCl2-induced HIF-1α expression correlates with proliferation and apoptosis in MKN-1 cells: A possible role for the PI3K/Akt pathway

  • Authors:
    • Tonang Dwi Ardyanto
    • Mitsuhiko Osaki
    • Naruo Tokuyasu
    • Yumi Nagahama
    • Hisao Ito

  • View Affiliations / Copyright

    Affiliations: Division of Organ Pathology, Department of Microbiology and Pathology, Faculty of Medicine, Tottori University, Tottori, Japan
  • Pages: 549-555
    |
    Published online on: September 1, 2006
       https://doi.org/10.3892/ijo.29.3.549
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Abstract

The exact mechanism behind the effect of hypoxia-inducible factor-1α (HIF-1α) on the proliferation and/or apoptosis of carcinoma cells is still a matter of debate. We treated a human gastric carcinoma cell line, MKN-1 (mutant P53), with 500 µM CoCl2. A dual-phase pattern of HIF-1α expression with an increase until 4 h followed by a decrease until 36 h was observed. Immunocytochemistry showed that nuclear translocation was maximal at 4 h of treatment, while trypan blue staining showed a dual-phase pattern. Instead of G1/S arrest, FACS showed an increase in the pre-G1 fraction and G2/M arrest that correlated with Cyclin-B1, SKP-2 and P27 expression. Starting at 6 h, the apoptotic index increased in a time-dependent manner, in correlation with the expression of HIF-1α, Bcl-2, Bcl-xL, Bax and cleaved-Caspase-9. Phosphorylation of Akt was inhibited by CoCl2 treatment and LY294002 treatment inhibited HIF-1α expression in a dose-dependent manner. These results suggested that the alteration of CoCl2-induced HIF-1α expression correlated with proliferation and apoptosis in MKN-1 cells. A possible role for the PI3K/Akt pathway was indicated in this model of hypoxia.

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Copy and paste a formatted citation
Spandidos Publications style
Ardyanto TD, Osaki M, Tokuyasu N, Nagahama Y and Ito H: CoCl2-induced HIF-1α expression correlates with proliferation and apoptosis in MKN-1 cells: A possible role for the PI3K/Akt pathway. Int J Oncol 29: 549-555, 2006.
APA
Ardyanto, T.D., Osaki, M., Tokuyasu, N., Nagahama, Y., & Ito, H. (2006). CoCl2-induced HIF-1α expression correlates with proliferation and apoptosis in MKN-1 cells: A possible role for the PI3K/Akt pathway. International Journal of Oncology, 29, 549-555. https://doi.org/10.3892/ijo.29.3.549
MLA
Ardyanto, T. D., Osaki, M., Tokuyasu, N., Nagahama, Y., Ito, H."CoCl2-induced HIF-1α expression correlates with proliferation and apoptosis in MKN-1 cells: A possible role for the PI3K/Akt pathway". International Journal of Oncology 29.3 (2006): 549-555.
Chicago
Ardyanto, T. D., Osaki, M., Tokuyasu, N., Nagahama, Y., Ito, H."CoCl2-induced HIF-1α expression correlates with proliferation and apoptosis in MKN-1 cells: A possible role for the PI3K/Akt pathway". International Journal of Oncology 29, no. 3 (2006): 549-555. https://doi.org/10.3892/ijo.29.3.549
Copy and paste a formatted citation
x
Spandidos Publications style
Ardyanto TD, Osaki M, Tokuyasu N, Nagahama Y and Ito H: CoCl2-induced HIF-1α expression correlates with proliferation and apoptosis in MKN-1 cells: A possible role for the PI3K/Akt pathway. Int J Oncol 29: 549-555, 2006.
APA
Ardyanto, T.D., Osaki, M., Tokuyasu, N., Nagahama, Y., & Ito, H. (2006). CoCl2-induced HIF-1α expression correlates with proliferation and apoptosis in MKN-1 cells: A possible role for the PI3K/Akt pathway. International Journal of Oncology, 29, 549-555. https://doi.org/10.3892/ijo.29.3.549
MLA
Ardyanto, T. D., Osaki, M., Tokuyasu, N., Nagahama, Y., Ito, H."CoCl2-induced HIF-1α expression correlates with proliferation and apoptosis in MKN-1 cells: A possible role for the PI3K/Akt pathway". International Journal of Oncology 29.3 (2006): 549-555.
Chicago
Ardyanto, T. D., Osaki, M., Tokuyasu, N., Nagahama, Y., Ito, H."CoCl2-induced HIF-1α expression correlates with proliferation and apoptosis in MKN-1 cells: A possible role for the PI3K/Akt pathway". International Journal of Oncology 29, no. 3 (2006): 549-555. https://doi.org/10.3892/ijo.29.3.549
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