Effects of RNAi-mediated Smad4 silencing on growth and apoptosis of human rhabdomyosarcoma cells

  • Authors:
    • Lv Ye
    • Hongying Zhang
    • Li Zhang
    • Guanghua Yang
    • Qi Ke
    • Hua Guo
    • Hong Bu
  • View Affiliations

  • Published online on: November 1, 2006     https://doi.org/10.3892/ijo.29.5.1149
  • Pages: 1149-1157
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Abstract

Transforming growth factor-β (TGF-β) signals through membrane-bound heteromeric serine/threonine kinase receptors. Upon ligand binding, TGF-β activates intracellular Smad proteins and regulates proliferation and apoptosis in various cell types. To demonstrate the effects of TGF-β/Smad signal on growth and apoptosis of human embryonal rhabdomyosarcoma (RMS) cells, a strategy of RNAi-mediated ‘gene silencing’ of Smad4 was used to interrupt endogenous TGF-β/Smad signaling in an RMS cell line, RD, and the regulation of exogenous TGF-β1 to growth and apoptosis of the cells was also determined. Physiologically, TGF-β/Smad signaling was essential for the normal growth of RD. The interruption of endogenous TGF-β/Smad signaling by RNAi significantly suppressed the growth of RD cells and dramatically induced apoptosis of RD cells. Exogenous TGF-β1 also inhibited the growth of RD cells, but had no effect on apoptosis. It also partially counteracted the growth inhibition and apoptosis induced by Smad4 silencing in RD cells. These findings provide a new insight into how TGF-β/Smad signaling regulates the growth and apoptosis of cancer cells. Moreover, as a powerful tool, shRNA interference suppresses endogenous Smad4 gene expression and subsequently modulates cell growth and apoptosis, which may provide a novel basis for the development of rational intervention strategies in RMS therapy.

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November 2006
Volume 29 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Ye L, Zhang H, Zhang L, Yang G, Ke Q, Guo H and Bu H: Effects of RNAi-mediated Smad4 silencing on growth and apoptosis of human rhabdomyosarcoma cells. Int J Oncol 29: 1149-1157, 2006.
APA
Ye, L., Zhang, H., Zhang, L., Yang, G., Ke, Q., Guo, H., & Bu, H. (2006). Effects of RNAi-mediated Smad4 silencing on growth and apoptosis of human rhabdomyosarcoma cells. International Journal of Oncology, 29, 1149-1157. https://doi.org/10.3892/ijo.29.5.1149
MLA
Ye, L., Zhang, H., Zhang, L., Yang, G., Ke, Q., Guo, H., Bu, H."Effects of RNAi-mediated Smad4 silencing on growth and apoptosis of human rhabdomyosarcoma cells". International Journal of Oncology 29.5 (2006): 1149-1157.
Chicago
Ye, L., Zhang, H., Zhang, L., Yang, G., Ke, Q., Guo, H., Bu, H."Effects of RNAi-mediated Smad4 silencing on growth and apoptosis of human rhabdomyosarcoma cells". International Journal of Oncology 29, no. 5 (2006): 1149-1157. https://doi.org/10.3892/ijo.29.5.1149