Reduced lecithin:retinol acyltransferase expression in human breast cancer

  • Authors:
    • Marni Sheren-Manoff
    • Sandra J. Shin
    • Dan Su
    • Dean Bok
    • Robert R. Rando
    • Lorraine J. Gudas
  • View Affiliations

  • Published online on: November 1, 2006     https://doi.org/10.3892/ijo.29.5.1193
  • Pages: 1193-1199
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Abstract

Retinoids, vitamin A (retinol) and related metabolites, have been shown to be important in regulating cell growth and differentiation. We have shown that expression of the enzyme lecithin:retinol acyltransferase (LRAT), which converts retinol to retinyl esters, is reduced in several human carcinomas as compared with adjacent normal tissue from the same organs. The purpose of this research was to determine if aspects of retinoid signaling are impaired in human breast cancer. We evaluated LRAT protein expression in neoplastic and adjacent, non-neoplastic glandular breast tissue specimens from human patients. We evaluated 26 specimens from patients diagnosed with breast cancer between 2003 and 2005. Representative paraffin-embedded tissue blocks from each tumor, with each containing adjacent non-neoplastic glandular breast tissue, were examined by immunohistochemistry with affinity purified antibodies to human LRAT protein. LRAT protein was prominently detected throughout the non-neoplastic glandular breast tissue in all of the specimens. Areas of ductal carcinoma in situ and well-differentiated invasive breast carcinomas showed an intensity of staining with the LRAT antibody which was similar to that of the adjacent normal tissue. Expression of LRAT protein progressively decreased with a reduction in the degree of tumor differentiation in invasive breast carcinomas. LRAT protein levels correlate better with the degree of ductal tumor differentiation than does estrogen receptor status in this study. Furthermore, normal human breast epithelium exhibits intense LRAT staining, indicating a major role for LRAT in human breast physiology.

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November 2006
Volume 29 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Sheren-Manoff M, Shin SJ, Su D, Bok D, Rando RR and Gudas LJ: Reduced lecithin:retinol acyltransferase expression in human breast cancer. Int J Oncol 29: 1193-1199, 2006.
APA
Sheren-Manoff, M., Shin, S.J., Su, D., Bok, D., Rando, R.R., & Gudas, L.J. (2006). Reduced lecithin:retinol acyltransferase expression in human breast cancer. International Journal of Oncology, 29, 1193-1199. https://doi.org/10.3892/ijo.29.5.1193
MLA
Sheren-Manoff, M., Shin, S. J., Su, D., Bok, D., Rando, R. R., Gudas, L. J."Reduced lecithin:retinol acyltransferase expression in human breast cancer". International Journal of Oncology 29.5 (2006): 1193-1199.
Chicago
Sheren-Manoff, M., Shin, S. J., Su, D., Bok, D., Rando, R. R., Gudas, L. J."Reduced lecithin:retinol acyltransferase expression in human breast cancer". International Journal of Oncology 29, no. 5 (2006): 1193-1199. https://doi.org/10.3892/ijo.29.5.1193