USE OF RHEK-1 IMMORTALIZED HUMAN KERATINOCYTES FOR DETECTION OF INDUCED MUTATION AT THE HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE LOCUS

  • Authors:
    • BL ALLEN-HOFFMANN
    • SJ SCHLOSSER
    • JS RHIM
  • View Affiliations

  • Published online on: October 1, 1993     https://doi.org/10.3892/ijo.3.4.619
  • Pages: 619-625
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Abstract

We report here the use of a nontumorigenic, immortalized human keratinocyte line, RHEK-1, for the detection of rare mutations induced at the hypoxanthine-guanine phosphoribosyltransferase locus. RHEK-1 keratinocytes were used as a prototype to determine mutagen treatment conditions, plating density, and phenotypic expression time for maximum recovery of thioguanine-resistant mutants. Mutation frequency was measured after exposure to ionizing radiation or to polycyclic aromatic hydrocarbons. 7,12-Dimethylbenz[a]-anthracene and benzo[a]pyrene caused almost no cytotoxicity, but induced thioguanine-resistant mutants at frequencies as much as 30 to 40-fold higher than the median spontaneous frequency of 7 x 10(-6). X-irradiation was also an efficient mutagen in RHEK-1 keratinocytes. The mutants were aminopterin-sensitive and possessed no measurable hypoxanthine-guanine phosphoribosyltransferase activity. The RHEK-1 human epithelial cell line is therefore useful for the study of induced mutation at a defined genetic locus as well as being an important model for the investigation, of molecular, cellular and genetic mechanisms of neoplastic transformation in human stratified squamous epithelia.

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October 1993
Volume 3 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
ALLEN-HOFFMANN B, SCHLOSSER S and RHIM J: USE OF RHEK-1 IMMORTALIZED HUMAN KERATINOCYTES FOR DETECTION OF INDUCED MUTATION AT THE HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE LOCUS. Int J Oncol 3: 619-625, 1993
APA
ALLEN-HOFFMANN, B., SCHLOSSER, S., & RHIM, J. (1993). USE OF RHEK-1 IMMORTALIZED HUMAN KERATINOCYTES FOR DETECTION OF INDUCED MUTATION AT THE HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE LOCUS. International Journal of Oncology, 3, 619-625. https://doi.org/10.3892/ijo.3.4.619
MLA
ALLEN-HOFFMANN, B., SCHLOSSER, S., RHIM, J."USE OF RHEK-1 IMMORTALIZED HUMAN KERATINOCYTES FOR DETECTION OF INDUCED MUTATION AT THE HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE LOCUS". International Journal of Oncology 3.4 (1993): 619-625.
Chicago
ALLEN-HOFFMANN, B., SCHLOSSER, S., RHIM, J."USE OF RHEK-1 IMMORTALIZED HUMAN KERATINOCYTES FOR DETECTION OF INDUCED MUTATION AT THE HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE LOCUS". International Journal of Oncology 3, no. 4 (1993): 619-625. https://doi.org/10.3892/ijo.3.4.619