BIOLOGICAL MARKERS IN TRANSURETHRAL BIOPSIES FROM BLADDER-CANCER - PRELIMINARY-RESULTS

  • Authors:
    • E BENINI
    • A COSTA
    • G PIZZOCARO
    • B CAMPO
    • A MILANI
    • T TORELLI
    • S VENERONI
    • G ORDESI
    • S PILOTTI
    • R SILVESTRINI
  • View Affiliations

  • Published online on: November 1, 1993     https://doi.org/10.3892/ijo.3.5.817
  • Pages: 817-821
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Abstract

A biologic profile including proliferative.activity, evaluated as H-3-thymidine labeling index (H-3-dT LI), DNA ploidy, p53 tumor-suppressor gene and P-glycoprotein (P-170), as an expression of the multidrug resistance gene, was defined for 50 primary transitional cell carcinomas of the bladder. H-3-dT LI was evaluated by autoradiography on histologic sections after incubation of fresh tumor biopsies with H-3-thymidine. Ploidy was defined by flow cytometric analysis of DNA content on nuclei suspensions obtained from frozen material. Expression of p53 protein and P-170 glycoprotein was detected by immunohistochemistry using the PAb1801 and C219 monoclonal antibody respectively, on sections from paraffin-embedded tumor biopsies. Invasive tumors showed a higher median H-3-dT LI (12.7% vs 4.2%) and a higher frequency of aneuploidy (73% vs 43%) and more frequently expressed p53 (82% vs 36%) than superficial tumors. Further analysis showed that proliferative activity was higher in invasive than in superficial cancers only in p53-positive or aneuploid tumors and not in p53-negative or diploid tumors. Moreover, proliferative activity and p53 overexpression, but not ploidy, were directly related to histologic grading and tumor stage. Generally, P-170 was not significantly related to any biologic or clinico-pathologic factor. Kinetic and phenotypic biologic markers are differently related to clinico-pathologic factors. A panel of biologic features can be easily evaluated on small transurethral biopsies at diagnosis, during endocavitary treatment or follow-up in bladder cancer patients.

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November 1993
Volume 3 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
BENINI E, COSTA A, PIZZOCARO G, CAMPO B, MILANI A, TORELLI T, VENERONI S, ORDESI G, PILOTTI S, SILVESTRINI R, SILVESTRINI R, et al: BIOLOGICAL MARKERS IN TRANSURETHRAL BIOPSIES FROM BLADDER-CANCER - PRELIMINARY-RESULTS. Int J Oncol 3: 817-821, 1993
APA
BENINI, E., COSTA, A., PIZZOCARO, G., CAMPO, B., MILANI, A., TORELLI, T. ... SILVESTRINI, R. (1993). BIOLOGICAL MARKERS IN TRANSURETHRAL BIOPSIES FROM BLADDER-CANCER - PRELIMINARY-RESULTS. International Journal of Oncology, 3, 817-821. https://doi.org/10.3892/ijo.3.5.817
MLA
BENINI, E., COSTA, A., PIZZOCARO, G., CAMPO, B., MILANI, A., TORELLI, T., VENERONI, S., ORDESI, G., PILOTTI, S., SILVESTRINI, R."BIOLOGICAL MARKERS IN TRANSURETHRAL BIOPSIES FROM BLADDER-CANCER - PRELIMINARY-RESULTS". International Journal of Oncology 3.5 (1993): 817-821.
Chicago
BENINI, E., COSTA, A., PIZZOCARO, G., CAMPO, B., MILANI, A., TORELLI, T., VENERONI, S., ORDESI, G., PILOTTI, S., SILVESTRINI, R."BIOLOGICAL MARKERS IN TRANSURETHRAL BIOPSIES FROM BLADDER-CANCER - PRELIMINARY-RESULTS". International Journal of Oncology 3, no. 5 (1993): 817-821. https://doi.org/10.3892/ijo.3.5.817