S-1 inhibits tumorigenicity and angiogenesis of human oral squamous cell carcinoma cells by suppressing expression of phosphorylated Akt, vascular endothelial growth factor and fibroblast growth factor-2

  • Authors:
    • Koji Harada
    • Supriatno
    • Yuichiro Kawashima
    • Hideo Yoshida
    • Mitsunobu Sato
  • View Affiliations

  • Published online on: February 1, 2007     https://doi.org/10.3892/ijo.30.2.365
  • Pages: 365-374
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Abstract

It has been reported that S-1 can exert antitumor effects on various human cancers including oral squamous cell carcinoma (OSCC). However, little is known about the detailed mechanisms of the antitumor activity of S-1. In the present study, we determined whether S-1 could suppress the angiogenesis and growth of human OSCC cells in vitro and in vivo. The S-1 component (5-FU plus CDHP) significantly suppressed the growth and migration of OSCC cells and BAEC, which inhibited tubule formation in HUVECs in vitro. Also, S-1 inhibited the nuclear factor-κB (NF-κB) activity in human OSCC cells in vitro. Moreover, S-1 inhibited the expression of survival signal, phosphorylated Akt (p-Akt), and of two major proangiogenic molecules, vascular endothelial growth factor (VEGF) and fibroblast growth factor-2 (FGF-2), in cells implanted into the subcutaneous tissue of nude mice. The decreased expression of p-Akt, VEGF and FGF-2 correlated with decreased tumorigenicity and decreased vascularization of lesions in vivo. These findings suggest that S-1 can suppress the angiogenesis and growth of OSCC cells by inhibiting the expression of p-Akt, VEGF and FGF-2 involved in the blockade of Akt/NF-κB pathway.

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February 2007
Volume 30 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Harada K, Supriatno , Kawashima Y, Yoshida H and Sato M: S-1 inhibits tumorigenicity and angiogenesis of human oral squamous cell carcinoma cells by suppressing expression of phosphorylated Akt, vascular endothelial growth factor and fibroblast growth factor-2. Int J Oncol 30: 365-374, 2007
APA
Harada, K., Supriatno, , Kawashima, Y., Yoshida, H., & Sato, M. (2007). S-1 inhibits tumorigenicity and angiogenesis of human oral squamous cell carcinoma cells by suppressing expression of phosphorylated Akt, vascular endothelial growth factor and fibroblast growth factor-2. International Journal of Oncology, 30, 365-374. https://doi.org/10.3892/ijo.30.2.365
MLA
Harada, K., Supriatno, , Kawashima, Y., Yoshida, H., Sato, M."S-1 inhibits tumorigenicity and angiogenesis of human oral squamous cell carcinoma cells by suppressing expression of phosphorylated Akt, vascular endothelial growth factor and fibroblast growth factor-2". International Journal of Oncology 30.2 (2007): 365-374.
Chicago
Harada, K., Supriatno, , Kawashima, Y., Yoshida, H., Sato, M."S-1 inhibits tumorigenicity and angiogenesis of human oral squamous cell carcinoma cells by suppressing expression of phosphorylated Akt, vascular endothelial growth factor and fibroblast growth factor-2". International Journal of Oncology 30, no. 2 (2007): 365-374. https://doi.org/10.3892/ijo.30.2.365