Transcriptomic profiling of radiation-resistant or -sensitive human colorectal cancer cells: Acute effects of a single X-radiation dose

  • Authors:
    • Cheng E. Ng
    • Qing Y. Liu
    • Sami S. Qutob
    • Bradley J. Scott
    • Wei L. Tan
    • Felix M. Mesak
  • View Affiliations

  • Published online on: June 1, 2007     https://doi.org/10.3892/ijo.30.6.1369
  • Pages: 1369-1380
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

We previously isolated several clones that were closely-related genetically from a human colorectal tumor (HCT116) cell line. These clones displayed significantly different X-radiation response phenotypes. In this paper, we investigated how a single dose of X-radiation modulated the transcriptomic profiles of either the radiation-resistant (HCT116Clone2_XRR) or the radiation-sensitive (HCT116CloneK_XRS) clone when each was compared to a reference clone, HCT116Clone10_control. The latter represented a control clone that displayed a similar X-radiation response as the parental HCT116 cells. Pooled RNAs were obtained from HCT116Clone2_XRR, HCT116CloneK_XRS or HCT116Clone10_control cells either before or at 10 min, 6 or 24 h after treatment with 4-Gy X-radiation. Transcriptomic profiles were assessed by cDNA microarrays. At least three independent experiments were carried out for each time point and statistical analysis was performed by paired t-test (p<0.05). From 19,200 genes/ESTs examined, we identified only 120 genes/ESTs that were differentially expressed at any one of these four time points. Interestingly, different patterns of gene modulation were observed between the radiation-sensitive and radiation-resistant clones. However, the fold changes of gene modulation were generally small (2-3 fold). Surprisingly, only 12.7% of 79 genes involved in DNA damage sensor/repair and cell cycle and between 2.6 and 9.2% of 76 genes involved in apoptosis, were significantly modulated in these early time points following irradiation. By comparison, up to 10% of 40 known housekeeping genes were differentially expressed. Thus in our experimental model, we were able to detect the up-regulation or down-regulation of mostly novel genes and/or pathways in the acute period (up to 24 h) following a single dose of 4-Gy X-radiation.

Related Articles

Journal Cover

June 2007
Volume 30 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Ng CE, Liu QY, Qutob SS, Scott BJ, Tan WL and Mesak FM: Transcriptomic profiling of radiation-resistant or -sensitive human colorectal cancer cells: Acute effects of a single X-radiation dose. Int J Oncol 30: 1369-1380, 2007.
APA
Ng, C.E., Liu, Q.Y., Qutob, S.S., Scott, B.J., Tan, W.L., & Mesak, F.M. (2007). Transcriptomic profiling of radiation-resistant or -sensitive human colorectal cancer cells: Acute effects of a single X-radiation dose. International Journal of Oncology, 30, 1369-1380. https://doi.org/10.3892/ijo.30.6.1369
MLA
Ng, C. E., Liu, Q. Y., Qutob, S. S., Scott, B. J., Tan, W. L., Mesak, F. M."Transcriptomic profiling of radiation-resistant or -sensitive human colorectal cancer cells: Acute effects of a single X-radiation dose". International Journal of Oncology 30.6 (2007): 1369-1380.
Chicago
Ng, C. E., Liu, Q. Y., Qutob, S. S., Scott, B. J., Tan, W. L., Mesak, F. M."Transcriptomic profiling of radiation-resistant or -sensitive human colorectal cancer cells: Acute effects of a single X-radiation dose". International Journal of Oncology 30, no. 6 (2007): 1369-1380. https://doi.org/10.3892/ijo.30.6.1369