LH-RH receptors in human colorectal cancers: Unexpected molecular targets for experimental therapy

  • Authors:
    • Karoly Szepeshazi
    • Andrew V. Schally
    • Gabor Halmos
  • View Affiliations

  • Published online on: June 1, 2007     https://doi.org/10.3892/ijo.30.6.1485
  • Pages: 1485-1492
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Abstract

Since the efficacy of chemotherapy can be enhanced by targeting to specific receptors on tumors, we investigated the expression of LH-RH receptors in 5 human colon cancer lines and the effects of cytotoxic LH-RH analogs on these tumors. Nude mice bearing HT-29, HCT-116, HCT-15, LoVo and Colo-320DM cancers were treated with cytotoxic LH-RH analogs AN-152 and AN-207 or their respective cytotoxic radicals doxorubicin (DOX) and 2-pyrrolino-DOX (AN-201). The reduction in tumor growth was evaluated, and cell proliferation characteristics as well as apoptosis were analyzed by histological methods. LH-RH receptors on the tumors were investigated by radioligand binding assays and their mRNA expression by reverse transcriptase-polymerase chain reaction (RT-PCR). All 5 colorectal cancer lines expressed high affinity binding sites for LH-RH, and mRNA for the LH-RH receptors. Both cytotoxic LH-RH analogs AN-152 and AN-207 powerfully inhibited growth of all colon cancers. AN-207 had the strongest effect on HT-29 and HCT-116 tumors, and AN-152 was the most effective on Colo-320DM cancers. Cytotoxic radicals AN-201 and DOX were less effective on these 3 tumors, but had effects similar to AN-152 and AN-207 on HCT-15 and LoVo carcinomas. The four cytotoxic compounds also differently affected apoptosis and proliferation rate of the various tumor lines. Our findings suggest that cytotoxic LH-RH analogs should be considered for the therapy of patients with advanced colorectal carcinoma.

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June 2007
Volume 30 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Szepeshazi K, Schally AV and Halmos G: LH-RH receptors in human colorectal cancers: Unexpected molecular targets for experimental therapy. Int J Oncol 30: 1485-1492, 2007
APA
Szepeshazi, K., Schally, A.V., & Halmos, G. (2007). LH-RH receptors in human colorectal cancers: Unexpected molecular targets for experimental therapy. International Journal of Oncology, 30, 1485-1492. https://doi.org/10.3892/ijo.30.6.1485
MLA
Szepeshazi, K., Schally, A. V., Halmos, G."LH-RH receptors in human colorectal cancers: Unexpected molecular targets for experimental therapy". International Journal of Oncology 30.6 (2007): 1485-1492.
Chicago
Szepeshazi, K., Schally, A. V., Halmos, G."LH-RH receptors in human colorectal cancers: Unexpected molecular targets for experimental therapy". International Journal of Oncology 30, no. 6 (2007): 1485-1492. https://doi.org/10.3892/ijo.30.6.1485