Overexpression of small ubiquitin-related modifier-1 and sumoylated Mdm2 in oral squamous cell carcinoma: Possible involvement in tumor proliferation and prognosis

Katayama,Akihiro; Ogino,Takeshi; Bandoh,Nobuyuki; Takahara,Miki; Kishibe,Kan; Nonaka,Satoshi; Harabuchi,Yasuaki

doi:10.3892/ijo.31.3.517

September 2007 Volume 31 Issue 3

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September 2007 Volume 31 Issue 3

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Article

Overexpression of small ubiquitin-related modifier-1 and sumoylated Mdm2 in oral squamous cell carcinoma: Possible involvement in tumor proliferation and prognosis

Authors:
- Akihiro Katayama
- Takeshi Ogino
- Nobuyuki Bandoh
- Miki Takahara
- Kan Kishibe
- Satoshi Nonaka
- Yasuaki Harabuchi
View Affiliations / Copyright

Affiliations: Department of Otolaryngology-Head and Neck Surgery, Asahikawa Medical College, Asahikawa 078-8510, Japan
Pages: 517-524
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Published online on: September 1, 2007

https://doi.org/10.3892/ijo.31.3.517
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Abstract

The purpose of this research was to identify a molecular clue to tumor proliferation in oral squamous cell carcinoma (SCC) and to test the value as a predictive marker for prognosis. In cDNA array analysis, small ubiquitin-related modifier-1 (SUMO-1) was expressed at much higher levels in oral SCC tissue and oral SCC cell lines than normal oral epithelium. The result was confirmed by RT-PCR analysis and Western blot analysis. Transfection of the anti-SUMO-1 antisense oligonucleotide to oral SCC cells significantly reduced proliferation of the cells. Immunoprecipitation and Western blot analyses revealed that the oncoprotein Mdm2 was present predominantly as a form of SUMO-1 congestion (sumoylation) rather than as a non-sumoylated form in both oral SCC tissues and cell lines. Immunohistological analysis revealed that patients who showed coexpression of SUMO-1 and Mdm2 experienced more frequently local recurrence after initial treatments. Multivariate analysis confirmed that the dual-high expression of SUMO-1 and Mdm2 was an independent factor for local failure. These result suggested that overexpression of Mdm2 caused by overexpression of SUMO-1 may be involved in tumor aggressiveness even in patients with early stage oral SCC. SUMO-1 may be useful as a novel target for therapy in oral SCC as well as a clinical indicator for tumor recurrence together with Mdm2.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Overexpression of small ubiquitin-related modifier-1 and sumoylated Mdm2 in oral squamous cell carcinoma: Possible involvement in tumor proliferation and prognosis

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Abstract

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