Upregulation of IGF-2 and IGF-1 receptor expression in oral cancer cell lines

  • Authors:
    • Garrett Brady
    • St John Crean
    • Prashant Naik
    • Supriya Kapas
  • View Affiliations

  • Published online on: October 1, 2007     https://doi.org/10.3892/ijo.31.4.875
  • Pages: 875-881
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Abstract

The insulin-like growth factors (IGFs) are a family of mitogenic proteins involved in the regulation of cell growth and differentiation. The presence and role of the IGF system in oral mucosal epithelium is not clear but could influence our understanding of the pathogenesis of oral cancer. We characterised the expression and function of IGF-1, IGF-2 and IGF receptor in human oral squamous carcinoma cell lines and normal oral epithelial cells as well as normal oral and squamous cell carcinoma tissues. Using reverse transcription followed by PCR, IGF-1 mRNA was only detected in normal cells, whereas IGF-2 and IGF-1R mRNA transcripts were highly expressed in tumour cell lines and tissues. Similar observations were seen by Western blot analysis and immunohistochemistry. Exogenous IGF-2, but not IGF-1, caused significant increases in DNA synthesis in the cell lines. IGF-2 also increased cell proliferation which was significantly attenuated in the presence of an IGF-2 neutralizing antibody or one which blocked IGF-1R. Taken together, these studies suggest that autocrine production of IGF-2, together with over-expression of IGF-1R, may be important components controlling the proliferation of oral carcinoma cells.

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October 2007
Volume 31 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Brady G, Crean SJ, Naik P and Kapas S: Upregulation of IGF-2 and IGF-1 receptor expression in oral cancer cell lines. Int J Oncol 31: 875-881, 2007
APA
Brady, G., Crean, S.J., Naik, P., & Kapas, S. (2007). Upregulation of IGF-2 and IGF-1 receptor expression in oral cancer cell lines. International Journal of Oncology, 31, 875-881. https://doi.org/10.3892/ijo.31.4.875
MLA
Brady, G., Crean, S. J., Naik, P., Kapas, S."Upregulation of IGF-2 and IGF-1 receptor expression in oral cancer cell lines". International Journal of Oncology 31.4 (2007): 875-881.
Chicago
Brady, G., Crean, S. J., Naik, P., Kapas, S."Upregulation of IGF-2 and IGF-1 receptor expression in oral cancer cell lines". International Journal of Oncology 31, no. 4 (2007): 875-881. https://doi.org/10.3892/ijo.31.4.875