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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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October 2007 Volume 31 Issue 4

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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October 2007 Volume 31 Issue 4

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Article

Differential expression of acidic proteins with progression in the MCF10 model of human breast disease

  • Authors:
    • Nathan S. Buchanan
    • Jia Zhao
    • Kan Zhu
    • Tasneem H. Patwa
    • Fred R. Miller
    • David M. Lubman

  • View Affiliations / Copyright

    Affiliations: Department of Chemistry, The University of Michigan, Ann Arbor, MI 48236, USA
  • Pages: 941-949
    |
    Published online on: October 1, 2007
       https://doi.org/10.3892/ijo.31.4.941
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Abstract

A proteomic characterization of one premalignant (MCF10AT1) and two malignant (MCF10CA1a and MCF10 CA1d) human breast cancer cell lines has been performed using a combination of two-dimensional liquid separations and mass spectrometry. Chromatofocusing (CF) and NPS-RP-HPLC are combined with ESI-TOF-MS to resolve and detect intact proteins. Simultaneously, fractions are collected and digested for protein identification using MALDI-MS peptide mass fingerprinting. Following protein identification a small database was compiled for use in comparison between IDs and measured masses taking into account variables such as pI, hydrophobicity and potential modifications. Out of 239 mass bands detected between pH 4.6 and 6.0, 133 have been definitively associated with identified proteins and 67 show consistent up/down regulation in two malignant breast cancer cell lines relative to the precursor premalignant cell line. Of these, 8 are also altered in the premalignant MCF10AT1 cell line by treatment with estradiol. Differentially expressed proteins indicate significant changes to the cytoskeleton, cellular metabolism, and adaptation to environmental stressors in malignant cell lines.

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Copy and paste a formatted citation
Spandidos Publications style
Buchanan NS, Zhao J, Zhu K, Patwa TH, Miller FR and Lubman DM: Differential expression of acidic proteins with progression in the MCF10 model of human breast disease. Int J Oncol 31: 941-949, 2007.
APA
Buchanan, N.S., Zhao, J., Zhu, K., Patwa, T.H., Miller, F.R., & Lubman, D.M. (2007). Differential expression of acidic proteins with progression in the MCF10 model of human breast disease. International Journal of Oncology, 31, 941-949. https://doi.org/10.3892/ijo.31.4.941
MLA
Buchanan, N. S., Zhao, J., Zhu, K., Patwa, T. H., Miller, F. R., Lubman, D. M."Differential expression of acidic proteins with progression in the MCF10 model of human breast disease". International Journal of Oncology 31.4 (2007): 941-949.
Chicago
Buchanan, N. S., Zhao, J., Zhu, K., Patwa, T. H., Miller, F. R., Lubman, D. M."Differential expression of acidic proteins with progression in the MCF10 model of human breast disease". International Journal of Oncology 31, no. 4 (2007): 941-949. https://doi.org/10.3892/ijo.31.4.941
Copy and paste a formatted citation
x
Spandidos Publications style
Buchanan NS, Zhao J, Zhu K, Patwa TH, Miller FR and Lubman DM: Differential expression of acidic proteins with progression in the MCF10 model of human breast disease. Int J Oncol 31: 941-949, 2007.
APA
Buchanan, N.S., Zhao, J., Zhu, K., Patwa, T.H., Miller, F.R., & Lubman, D.M. (2007). Differential expression of acidic proteins with progression in the MCF10 model of human breast disease. International Journal of Oncology, 31, 941-949. https://doi.org/10.3892/ijo.31.4.941
MLA
Buchanan, N. S., Zhao, J., Zhu, K., Patwa, T. H., Miller, F. R., Lubman, D. M."Differential expression of acidic proteins with progression in the MCF10 model of human breast disease". International Journal of Oncology 31.4 (2007): 941-949.
Chicago
Buchanan, N. S., Zhao, J., Zhu, K., Patwa, T. H., Miller, F. R., Lubman, D. M."Differential expression of acidic proteins with progression in the MCF10 model of human breast disease". International Journal of Oncology 31, no. 4 (2007): 941-949. https://doi.org/10.3892/ijo.31.4.941
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