GSH loss per se does not affect neuroblastoma survival and is not genotoxic

  • Authors:
    • Barbara Marengo
    • Emanuela Balbis
    • Stefania Patriarca
    • Chiara De Ciucis
    • Annalisa Furfaro
    • Mariapaola Nitti
    • Umberto Maria Marinari
    • Maria Adelaide Pronzato
    • Nicola Traverso
    • Cinzia Domenicotti
  • View Affiliations

  • Published online on: January 1, 2008     https://doi.org/10.3892/ijo.32.1.121
  • Pages: 121-127
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Depletion of glutathione (GSH) by buthionine sulfoximine (BSO) has been reported to be toxic against some cancer cells and to sensitize many tumours including neuroblastoma (NB) to anticancer drugs. The balance between the production rate of reactive oxygen species (ROS) and the function of GSH affects the intracellular reduction-oxidation status, which is crucial for the regulation of several cellular physiological functions. To assess the role of glutathione in neuroblastoma therapy, the effect of sublethal concentrations of BSO was studied in a panel of neuroblastoma cell lines characterized by different MYCN status. We found that GSH depletion per se not accompanied by ROS overproduction, does not affect cell survival, and is not genotoxic but induces HO-1 expression in GI-ME-N cell line, a representative example of MYCN non-amplified NB cells, having the highest basal levels of GSH among the tested NB lines. These observations might open a novel therapeutic window based on the possibility of modulating the cellular ‘activity’ of GSH.

Related Articles

Journal Cover

January 2008
Volume 32 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Marengo B, Balbis E, Patriarca S, De Ciucis C, Furfaro A, Nitti M, Marinari UM, Pronzato MA, Traverso N, Domenicotti C, Domenicotti C, et al: GSH loss per se does not affect neuroblastoma survival and is not genotoxic. Int J Oncol 32: 121-127, 2008
APA
Marengo, B., Balbis, E., Patriarca, S., De Ciucis, C., Furfaro, A., Nitti, M. ... Domenicotti, C. (2008). GSH loss per se does not affect neuroblastoma survival and is not genotoxic. International Journal of Oncology, 32, 121-127. https://doi.org/10.3892/ijo.32.1.121
MLA
Marengo, B., Balbis, E., Patriarca, S., De Ciucis, C., Furfaro, A., Nitti, M., Marinari, U. M., Pronzato, M. A., Traverso, N., Domenicotti, C."GSH loss per se does not affect neuroblastoma survival and is not genotoxic". International Journal of Oncology 32.1 (2008): 121-127.
Chicago
Marengo, B., Balbis, E., Patriarca, S., De Ciucis, C., Furfaro, A., Nitti, M., Marinari, U. M., Pronzato, M. A., Traverso, N., Domenicotti, C."GSH loss per se does not affect neuroblastoma survival and is not genotoxic". International Journal of Oncology 32, no. 1 (2008): 121-127. https://doi.org/10.3892/ijo.32.1.121