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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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February 2008 Volume 32 Issue 2

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Article

Serum starvation induces G1 arrest through suppression of skp2-CDK2 and CDK4 in SK-OV-3 cells

  • Authors:
    • Jae-Sik Shin
    • Seung-Woo Hong
    • Sae-Lo Oom Lee
    • Tae-Hee Kim
    • In-Chul Park
    • Sung-Kwan An
    • Won-Keun Lee
    • Jong-Seok Lim
    • Keun-Il Kim
    • Young Yang
    • Soo-Suk Lee
    • Dong-Hoon Jin
    • Myeong-Sok Lee

  • View Affiliations / Copyright

    Affiliations: Research Center for Women's Diseases, Division of Biological Sciences, Sookmyung Women's University, Seoul 140-742, Korea
  • Pages: 435-439
    |
    Published online on: February 1, 2008
       https://doi.org/10.3892/ijo.32.2.435
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Abstract

Recent studies have suggested that Skp2, an SCF-type ubiquitin ligase, positively regulates cell cycle through degradation of p27, which is an inhibitor of cyclin-dependent kinase 2 (CDK2), which drives cells from the G1 to S phase of cell cycles. In the present study, we examined key regulatory proteins involved in serum starvation-induced cell cycle arrest in human ovarian cancer cells, SK-OV-3. Cell cycle analysis showed that cells were arrested at the G1 phase after serum starvation. Western blot analysis showed that the protein levels of CDK4 and CDK2 were significantly decreased in SK-OV-3 cells. Consistently, Roscovitine, an inhibitor of CDK2, induced cell cycle arrest in normally proliferating cells and a chemical inhibitor of CDK4, 3-ATA [3-Amino-9-thio(10H)-acridone], was found to induce growth arrest. We also found that the protein level of Skp2 was dramatically decreased in response to serum starvation. Moreover, CDK2 protein, which allows cell cycle transit from the G1 to the S phase, was decreased when the Skp2 expression was inhibited by specific siRNA of Skp2, but CDK4 was not decreased. Therefore, these results suggest that serum starvation induces G1 arrest through suppression of Skp2-dependent CDK2 activity and Skp2-independent CDK4 activity in human SK-OV-3 ovarian cancer cells.

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Copy and paste a formatted citation
Spandidos Publications style
Shin J, Hong S, Lee SO, Kim T, Park I, An S, Lee W, Lim J, Kim K, Yang Y, Yang Y, et al: Serum starvation induces G1 arrest through suppression of skp2-CDK2 and CDK4 in SK-OV-3 cells. Int J Oncol 32: 435-439, 2008.
APA
Shin, J., Hong, S., Lee, S.O., Kim, T., Park, I., An, S. ... Lee, M. (2008). Serum starvation induces G1 arrest through suppression of skp2-CDK2 and CDK4 in SK-OV-3 cells. International Journal of Oncology, 32, 435-439. https://doi.org/10.3892/ijo.32.2.435
MLA
Shin, J., Hong, S., Lee, S. O., Kim, T., Park, I., An, S., Lee, W., Lim, J., Kim, K., Yang, Y., Lee, S., Jin, D., Lee, M."Serum starvation induces G1 arrest through suppression of skp2-CDK2 and CDK4 in SK-OV-3 cells". International Journal of Oncology 32.2 (2008): 435-439.
Chicago
Shin, J., Hong, S., Lee, S. O., Kim, T., Park, I., An, S., Lee, W., Lim, J., Kim, K., Yang, Y., Lee, S., Jin, D., Lee, M."Serum starvation induces G1 arrest through suppression of skp2-CDK2 and CDK4 in SK-OV-3 cells". International Journal of Oncology 32, no. 2 (2008): 435-439. https://doi.org/10.3892/ijo.32.2.435
Copy and paste a formatted citation
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Spandidos Publications style
Shin J, Hong S, Lee SO, Kim T, Park I, An S, Lee W, Lim J, Kim K, Yang Y, Yang Y, et al: Serum starvation induces G1 arrest through suppression of skp2-CDK2 and CDK4 in SK-OV-3 cells. Int J Oncol 32: 435-439, 2008.
APA
Shin, J., Hong, S., Lee, S.O., Kim, T., Park, I., An, S. ... Lee, M. (2008). Serum starvation induces G1 arrest through suppression of skp2-CDK2 and CDK4 in SK-OV-3 cells. International Journal of Oncology, 32, 435-439. https://doi.org/10.3892/ijo.32.2.435
MLA
Shin, J., Hong, S., Lee, S. O., Kim, T., Park, I., An, S., Lee, W., Lim, J., Kim, K., Yang, Y., Lee, S., Jin, D., Lee, M."Serum starvation induces G1 arrest through suppression of skp2-CDK2 and CDK4 in SK-OV-3 cells". International Journal of Oncology 32.2 (2008): 435-439.
Chicago
Shin, J., Hong, S., Lee, S. O., Kim, T., Park, I., An, S., Lee, W., Lim, J., Kim, K., Yang, Y., Lee, S., Jin, D., Lee, M."Serum starvation induces G1 arrest through suppression of skp2-CDK2 and CDK4 in SK-OV-3 cells". International Journal of Oncology 32, no. 2 (2008): 435-439. https://doi.org/10.3892/ijo.32.2.435
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