Kinetics and subcellular localization of 5-ALA-induced PpIX in DHL cells via two-photon excitation fluorescence microscopy

  • Authors:
    • Rong Chen
    • Zufang Huang
    • Guannan Chen
    • Yongzeng Li
    • Xianlian Chen
    • Jianxin Chen
    • Haishan Zeng
  • View Affiliations

  • Published online on: April 1, 2008     https://doi.org/10.3892/ijo.32.4.861
  • Pages: 861-867
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Abstract

Two-photon excitation fluorescence (TPEF) microscopy was used to measure the 5-aminolevulinic acid (5-ALA)-induced PpIX fluorescence in follicular lymphoma DHL cells. Kinetics of 5-ALA-induced PpIX accumulation in DHL cells under various 5-ALA concentrations was studied. We found that during the course of continuous incubation with 5-ALA, the relationship between the DHL cell fluorescence signal and the incubation time showed a biphasic variation. Initially the PpIX signal increased with the incubation time and reached the maximal value at about 3 h, and then it decreased with time during the subsequent incubation period. By labeling the 5-ALA incubated DHL cells with different organelle-specific fluorescence probes: Rhodamine 123 (for mitochondria), DioC6(3) (for endoplasmic reticulum) and LysoTracker Green (for lysosomes) respectively, we found that 5-ALA-induced PpIX was primarily localized in endoplasmic reticulum and mitochondria; its concentration in the lysosome was much lower. The results suggested that 5-ALA could potentially be an effective photosensitizer in photodynamic purging of DHL cells. Two-photon excitation fluorescence microscope is a useful tool for studying 5-ALA-induced PpIX subcellular localization.

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April 2008
Volume 32 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Chen R, Huang Z, Chen G, Li Y, Chen X, Chen J and Zeng H: Kinetics and subcellular localization of 5-ALA-induced PpIX in DHL cells via two-photon excitation fluorescence microscopy. Int J Oncol 32: 861-867, 2008
APA
Chen, R., Huang, Z., Chen, G., Li, Y., Chen, X., Chen, J., & Zeng, H. (2008). Kinetics and subcellular localization of 5-ALA-induced PpIX in DHL cells via two-photon excitation fluorescence microscopy. International Journal of Oncology, 32, 861-867. https://doi.org/10.3892/ijo.32.4.861
MLA
Chen, R., Huang, Z., Chen, G., Li, Y., Chen, X., Chen, J., Zeng, H."Kinetics and subcellular localization of 5-ALA-induced PpIX in DHL cells via two-photon excitation fluorescence microscopy". International Journal of Oncology 32.4 (2008): 861-867.
Chicago
Chen, R., Huang, Z., Chen, G., Li, Y., Chen, X., Chen, J., Zeng, H."Kinetics and subcellular localization of 5-ALA-induced PpIX in DHL cells via two-photon excitation fluorescence microscopy". International Journal of Oncology 32, no. 4 (2008): 861-867. https://doi.org/10.3892/ijo.32.4.861