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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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May 2008 Volume 32 Issue 5

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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May 2008 Volume 32 Issue 5

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Article

Pleiotropic effects of fenretinide in neuroblastoma cell lines and multicellular tumor spheroids

  • Authors:
    • Roos Cuperus
    • Godelieve A.M. Tytgat
    • René Leen
    • Pedro Brites
    • Johannes Bras
    • Huib N. Caron
    • André B.P. Van Kuilenburg

  • View Affiliations / Copyright

    Affiliations: Laboratory Genetic Metabolic Diseases and Department of Pediatrics/Emma Children's Hospital, Academic Medical Centre, University of Amsterdam, 1100 DE Amsterdam, The Netherlands
  • Pages: 1011-1019
    |
    Published online on: May 1, 2008
       https://doi.org/10.3892/ijo.32.5.1011
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Abstract

The efficacy and mechanism of action of fenretinide (4-HPR), a vitamin A analogue, was investigated in a panel of six neuroblastoma cell lines and multicellular tumor spheroids. The latter are three dimensional cell aggregates and as such, a model for micrometastases. In all cell lines, the production of reactive oxygen species (ROS) increased with 163-680% after 1 h of treatment with 4-HPR. In addition, a decrease of the mitochondrial membrane potential of 30-75% was observed after 4 h of incubation with 4-HPR. A 6-12-fold difference was observed between the IC50 values for cell proliferation and viability between the most sensitive (IMR32) and most resistant (NASS) cell line towards 4-HPR. Flow cytometric analysis showed an increased amount of apoptotic bodies and no cell-cycle arrest. The antioxidant Trolox completely inhibited the accumulation of 4HPR-induced ROS and prevented the 4HPR-associated cytotoxicity. In all neuroblastoma spheroids, 4-HPR induced a complete cytostasis at clinical relevant concentrations (3-10 µM). Immunohistochemical analysis of 4-HPR-treated spheroids showed a decreased staining for proliferation marker Ki-67 and an increased staining for cleaved-PARP, a marker of apoptosis. Our results suggest that 4-HPR might be a promising agent for the treatment of micrometastases and high-risk neuroblastoma.

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Copy and paste a formatted citation
Spandidos Publications style
Cuperus R, Tytgat GA, Leen R, Brites P, Bras J, Caron HN and Van Kuilenburg AB: Pleiotropic effects of fenretinide in neuroblastoma cell lines and multicellular tumor spheroids. Int J Oncol 32: 1011-1019, 2008.
APA
Cuperus, R., Tytgat, G.A., Leen, R., Brites, P., Bras, J., Caron, H.N., & Van Kuilenburg, A.B. (2008). Pleiotropic effects of fenretinide in neuroblastoma cell lines and multicellular tumor spheroids. International Journal of Oncology, 32, 1011-1019. https://doi.org/10.3892/ijo.32.5.1011
MLA
Cuperus, R., Tytgat, G. A., Leen, R., Brites, P., Bras, J., Caron, H. N., Van Kuilenburg, A. B."Pleiotropic effects of fenretinide in neuroblastoma cell lines and multicellular tumor spheroids". International Journal of Oncology 32.5 (2008): 1011-1019.
Chicago
Cuperus, R., Tytgat, G. A., Leen, R., Brites, P., Bras, J., Caron, H. N., Van Kuilenburg, A. B."Pleiotropic effects of fenretinide in neuroblastoma cell lines and multicellular tumor spheroids". International Journal of Oncology 32, no. 5 (2008): 1011-1019. https://doi.org/10.3892/ijo.32.5.1011
Copy and paste a formatted citation
x
Spandidos Publications style
Cuperus R, Tytgat GA, Leen R, Brites P, Bras J, Caron HN and Van Kuilenburg AB: Pleiotropic effects of fenretinide in neuroblastoma cell lines and multicellular tumor spheroids. Int J Oncol 32: 1011-1019, 2008.
APA
Cuperus, R., Tytgat, G.A., Leen, R., Brites, P., Bras, J., Caron, H.N., & Van Kuilenburg, A.B. (2008). Pleiotropic effects of fenretinide in neuroblastoma cell lines and multicellular tumor spheroids. International Journal of Oncology, 32, 1011-1019. https://doi.org/10.3892/ijo.32.5.1011
MLA
Cuperus, R., Tytgat, G. A., Leen, R., Brites, P., Bras, J., Caron, H. N., Van Kuilenburg, A. B."Pleiotropic effects of fenretinide in neuroblastoma cell lines and multicellular tumor spheroids". International Journal of Oncology 32.5 (2008): 1011-1019.
Chicago
Cuperus, R., Tytgat, G. A., Leen, R., Brites, P., Bras, J., Caron, H. N., Van Kuilenburg, A. B."Pleiotropic effects of fenretinide in neuroblastoma cell lines and multicellular tumor spheroids". International Journal of Oncology 32, no. 5 (2008): 1011-1019. https://doi.org/10.3892/ijo.32.5.1011
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