Inverse relationship between E-cadherin and p27Kip1 expression in renal cell carcinoma

  • Authors:
    • Toshiro Migita
    • Yoshinao Oda
    • Katsuaki Masuda
    • Akira Hirata
    • Michihiko Kuwano
    • Seiji Naito
    • Masazumi Tsuneyoshi
  • View Affiliations

  • Published online on: July 1, 2008     https://doi.org/10.3892/ijo.33.1.41
  • Pages: 41-47
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Abstract

The cell-cell adhesion system plays a pivotal role in the maintenance of tissue structure and cell-cell communication. E-cadherin is a major adhesion protein of the epithelial cells, and E-cadherin expression may be involved in the regulation of cell proliferation or differentiation. To address the relationship between cell-cell adhesion and cell proliferation, we focused on the alteration of p27Kip1 (p27), a cyclin-dependent kinase inhibitor, by E-cadherin-mediated adhesion. In an immunohistochemical study of 76 cases of renal cell carcinoma (RCC), the p27-labeling index (LI) was 67% in E-cadherin-reduced RCC, but only 28% in E-cadherin-preserved RCC. E-cadherin-expressing cells rarely expressed p27 in various cancers including those of the breast, colon, liver and prostate. In a subconfluent monolayer culture, the E-cadherin levels were increased steadily in the E-cadherin positive RCC cell line ACHN, whereas the p27 levels were decreased. Subsequent exposure of ACHN cells to the E-cadherin-specific function-blocking antibody reduced the growth associated with the increase in p27 and the decrease in phosphorylated epidermal growth factor receptor (EGFR). In the E-cadherin negative RCC cell line Caki-1, these effects were not observed. These results suggest that E-cadherin-mediated adhesion may be involved in the contact stimulation for cell proliferation in part through the downregulation of p27 and the activation of EGFR in human cancers.

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July 2008
Volume 33 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Migita T, Oda Y, Masuda K, Hirata A, Kuwano M, Naito S and Tsuneyoshi M: Inverse relationship between E-cadherin and p27Kip1 expression in renal cell carcinoma. Int J Oncol 33: 41-47, 2008.
APA
Migita, T., Oda, Y., Masuda, K., Hirata, A., Kuwano, M., Naito, S., & Tsuneyoshi, M. (2008). Inverse relationship between E-cadherin and p27Kip1 expression in renal cell carcinoma. International Journal of Oncology, 33, 41-47. https://doi.org/10.3892/ijo.33.1.41
MLA
Migita, T., Oda, Y., Masuda, K., Hirata, A., Kuwano, M., Naito, S., Tsuneyoshi, M."Inverse relationship between E-cadherin and p27Kip1 expression in renal cell carcinoma". International Journal of Oncology 33.1 (2008): 41-47.
Chicago
Migita, T., Oda, Y., Masuda, K., Hirata, A., Kuwano, M., Naito, S., Tsuneyoshi, M."Inverse relationship between E-cadherin and p27Kip1 expression in renal cell carcinoma". International Journal of Oncology 33, no. 1 (2008): 41-47. https://doi.org/10.3892/ijo.33.1.41