THE ANTIPROLIFERATIVE RIMINOPHENAZINE AGENTS CLOFAZIMINE AND B669 PROMOTE LYSOPHOSPHOLIPID-MEDIATED INHIBITION OF NA+, K+-ADENOSINE TRIPHOSPHATASE-ACTIVITY IN CANCER CELL-LINES IN-VITRO

  • Authors:
    • CEJ VANRENSBURG
    • EE DEBRUYN
    • R ANDERSON
  • View Affiliations

  • Published online on: May 1, 1994     https://doi.org/10.3892/ijo.4.5.1115
  • Pages: 1115-1119
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Abstract

The effects of the anti-proliferative, phospholipase A(2) (PLA(2))-activating riminophenazine agents, clofazimine and B669, on the Na+, K+-adenosine triphosphatase activity of the FaDu human pharynx squamous carcinoma cell line have been investigated in vitro. At concentrations of 1.25-10 mu g/ml both agents caused dose-related enhancement of PLA(2), as measured by increased release of lysophosphatidylcholine (LPC), and inhibition of Na+, K+-ATPase in intact cells and isolated membrane preparations. The inhibitory effects of both riminophenazines on the Na+, K+-ATPase activity of FaDu cells were mimicked by reagent LPC and prevented by treatment of the cells with the lysophospholipid-neutralizing agents alpha-tocopherol and lysophospholipase. Riminophenazine-mediated inhibition of Na+, K+-ATPase activity was also observed with the HeLa (human cervix epitheloid carcinoma) and T24 (human transitional cell bladder carcinoma) cell lines. The anti-proliferative activity of clofazimine and B669 is therefore probably achieved by lysophospholipid-mediated inactivation of Na+, K+-ATPase.

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May 1994
Volume 4 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
VANRENSBURG C, DEBRUYN E and ANDERSON R: THE ANTIPROLIFERATIVE RIMINOPHENAZINE AGENTS CLOFAZIMINE AND B669 PROMOTE LYSOPHOSPHOLIPID-MEDIATED INHIBITION OF NA+, K+-ADENOSINE TRIPHOSPHATASE-ACTIVITY IN CANCER CELL-LINES IN-VITRO. Int J Oncol 4: 1115-1119, 1994
APA
VANRENSBURG, C., DEBRUYN, E., & ANDERSON, R. (1994). THE ANTIPROLIFERATIVE RIMINOPHENAZINE AGENTS CLOFAZIMINE AND B669 PROMOTE LYSOPHOSPHOLIPID-MEDIATED INHIBITION OF NA+, K+-ADENOSINE TRIPHOSPHATASE-ACTIVITY IN CANCER CELL-LINES IN-VITRO. International Journal of Oncology, 4, 1115-1119. https://doi.org/10.3892/ijo.4.5.1115
MLA
VANRENSBURG, C., DEBRUYN, E., ANDERSON, R."THE ANTIPROLIFERATIVE RIMINOPHENAZINE AGENTS CLOFAZIMINE AND B669 PROMOTE LYSOPHOSPHOLIPID-MEDIATED INHIBITION OF NA+, K+-ADENOSINE TRIPHOSPHATASE-ACTIVITY IN CANCER CELL-LINES IN-VITRO". International Journal of Oncology 4.5 (1994): 1115-1119.
Chicago
VANRENSBURG, C., DEBRUYN, E., ANDERSON, R."THE ANTIPROLIFERATIVE RIMINOPHENAZINE AGENTS CLOFAZIMINE AND B669 PROMOTE LYSOPHOSPHOLIPID-MEDIATED INHIBITION OF NA+, K+-ADENOSINE TRIPHOSPHATASE-ACTIVITY IN CANCER CELL-LINES IN-VITRO". International Journal of Oncology 4, no. 5 (1994): 1115-1119. https://doi.org/10.3892/ijo.4.5.1115