A NOVEL V-ERB-B-TRANSFORMED CELL-LINE CO-EXPRESSING LYMPHOID AND MYELOID SURFACE-MARKERS
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- Published online on: June 1, 1994 https://doi.org/10.3892/ijo.4.6.1277
- Pages: 1277-1281
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Abstract
We have generated a novel hematopoietic cell line which co-expresses lymphoid and myeloid cell surface markers. This cell line, termed E6-2TH, was derived from thymus tissue of a mouse which developed leukemia following lethal irradiation and reconstitution with retrovirally infected bone marrow progenitor cells. Infection was effected with a recombinant murine retrovirus containing v-erb-B. The cells which grew out from the thymus tissue were initially dependent on WEHI-3B conditioned medium as a source of exogenous growth factor, but progressed in culture to become growth factor independent. Flow cytometric analysis using lineage-specific surface markers demonstrated that the initial growth factor dependent cells co-expressed myeloid and B lymphoid antigens, while the derived growth factor independent cells expressed only antigens specific to the B lymphoid lineage. This result indicates a mixed lymphoid/myeloid phenotype in this cell line and subsequent lineage specialisation during in vitro tumor progression.