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CELL-CYCLE KINETICS OF PERMANENT GLIOMA-CELLS MEASURED BY PULSE LABELING WITH BROMODEOXYURIDINE

  • Authors:
    • F FOGT
    • AO VORTMEYER
    • SR TAHAN
    • D STAVROU
    • NW ISTFAN

  • View Affiliations

  • Published online on: June 1, 1994     https://doi.org/10.3892/ijo.4.6.1315
  • Pages: 1315-1321
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Abstract

Cell phase distribution and cycle kinetics of six human glioblastoma cell lines were characterized after labelling with 5-Bromo-2-deoxyuridine (BrdUrd). Cycle time (T(c)), DNA synthesis time (T(s)), and potential doubling time (T(pot)) were compared with the actual doubling time (T(d)) of the growing cell population. Mathematical estimates closely correlated with T(d). Low labelling index (LI) correlated with short T(s) and vice versa. T(s) and LI allowed grouping of the cell lines in two clusters. The mean number of silver stained nucleolar organizer regions (mAgNORs) and percentage of cells with more than five AgNORs (pAgNOR) were counted. AgNORs closely related to LI. Low mAgNORs and pAgNORs correlated with fast T(s) among the clustered cell lines.

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June 1994
Volume 4 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
FOGT F, VORTMEYER A, TAHAN S, STAVROU D and ISTFAN N: CELL-CYCLE KINETICS OF PERMANENT GLIOMA-CELLS MEASURED BY PULSE LABELING WITH BROMODEOXYURIDINE. Int J Oncol 4: 1315-1321, 1994
APA
FOGT, F., VORTMEYER, A., TAHAN, S., STAVROU, D., & ISTFAN, N. (1994). CELL-CYCLE KINETICS OF PERMANENT GLIOMA-CELLS MEASURED BY PULSE LABELING WITH BROMODEOXYURIDINE. International Journal of Oncology, 4, 1315-1321. https://doi.org/10.3892/ijo.4.6.1315
MLA
FOGT, F., VORTMEYER, A., TAHAN, S., STAVROU, D., ISTFAN, N."CELL-CYCLE KINETICS OF PERMANENT GLIOMA-CELLS MEASURED BY PULSE LABELING WITH BROMODEOXYURIDINE". International Journal of Oncology 4.6 (1994): 1315-1321.
Chicago
FOGT, F., VORTMEYER, A., TAHAN, S., STAVROU, D., ISTFAN, N."CELL-CYCLE KINETICS OF PERMANENT GLIOMA-CELLS MEASURED BY PULSE LABELING WITH BROMODEOXYURIDINE". International Journal of Oncology 4, no. 6 (1994): 1315-1321. https://doi.org/10.3892/ijo.4.6.1315