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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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September 1994 Volume 5 Issue 3

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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September 1994 Volume 5 Issue 3

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Article

PCR AMPLIFICATION OF 40-YEAR-OLD PARAFFIN-EMBEDDED TUMOR-TISSUES - COMPARISON OF 4 DIFFERENT DNA EXTRACTION AND PURIFICATION METHODS

  • Authors:
    • WG WANG
    • P KUMAR
    • M SCHWARZ
    • G MALONE
    • A HAWORTH
    • S KUMAR

  • View Affiliations / Copyright

    Affiliations: MANCHESTER METROPOLITAN UNIV,DEPT BIOL SCI,MANCHESTER M1 5GD,LANCS,ENGLAND. ROYAL MANCHESTER CHILDRENS HOSP,REG MOLEC GENET LAB,MANCHESTER M27 4HA,LANCS,ENGLAND. CHRISTIE HOSP & HOLT RADIUM INST,CLIN RES LABS,TUMOR BIOL LAB,MANCHESTER,LANCS,ENGLAND.
  • Pages: 453-457
    |
    Published online on: September 1, 1994
       https://doi.org/10.3892/ijo.5.3.453
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Abstract

Four different methods of DNA extraction from formalin-fixed, paraffin-embedded tissues were compared for their ability to produce DNA suitable as a template for polymerase chain reaction (PCR). Seven paraffin-embedded rhabdomyosarcoma tissue blocks from the 1950s and one from 1960 were treated with the following extraction methods: 1. Proteinase K digestion and phenol/chloroform extraction; 2. Proteinase K digestion followed by boiling to inactivate the enzyme; 3. Proteinase K digestion, addition of Chelex-100, followed by boiling; and 4. Proteinase K digestion and Prep-A-Gene purification. DNA extracted by methods 1 and 2 was degraded, but DNA of high molecular weight was recovered in every sample extracted by methods 3 and 4 - even though some degradation was observed. Extracted DNA was used as a template for PCR amplification of exon 4 of the PAX-3 gene. PCR was successful in 7 out of 8 samples prepared using methods 3 and 4, producing levels of amplified product equivalent to those obtained with control DNA obtained from fresh lymphocytes. Only very weak products were found in samples prepared by methods 1 (2 out of 8) and 2 (4 out of 8). These results indicate that chelation of polyvalent ions (Chelex-100 method) or obviating the need for boiling (Prep-A-Gene) may protect DNA during extraction.

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Copy and paste a formatted citation
Spandidos Publications style
WANG W, KUMAR P, SCHWARZ M, MALONE G, HAWORTH A and KUMAR S: PCR AMPLIFICATION OF 40-YEAR-OLD PARAFFIN-EMBEDDED TUMOR-TISSUES - COMPARISON OF 4 DIFFERENT DNA EXTRACTION AND PURIFICATION METHODS. Int J Oncol 5: 453-457, 1994.
APA
WANG, W., KUMAR, P., SCHWARZ, M., MALONE, G., HAWORTH, A., & KUMAR, S. (1994). PCR AMPLIFICATION OF 40-YEAR-OLD PARAFFIN-EMBEDDED TUMOR-TISSUES - COMPARISON OF 4 DIFFERENT DNA EXTRACTION AND PURIFICATION METHODS. International Journal of Oncology, 5, 453-457. https://doi.org/10.3892/ijo.5.3.453
MLA
WANG, W., KUMAR, P., SCHWARZ, M., MALONE, G., HAWORTH, A., KUMAR, S."PCR AMPLIFICATION OF 40-YEAR-OLD PARAFFIN-EMBEDDED TUMOR-TISSUES - COMPARISON OF 4 DIFFERENT DNA EXTRACTION AND PURIFICATION METHODS". International Journal of Oncology 5.3 (1994): 453-457.
Chicago
WANG, W., KUMAR, P., SCHWARZ, M., MALONE, G., HAWORTH, A., KUMAR, S."PCR AMPLIFICATION OF 40-YEAR-OLD PARAFFIN-EMBEDDED TUMOR-TISSUES - COMPARISON OF 4 DIFFERENT DNA EXTRACTION AND PURIFICATION METHODS". International Journal of Oncology 5, no. 3 (1994): 453-457. https://doi.org/10.3892/ijo.5.3.453
Copy and paste a formatted citation
x
Spandidos Publications style
WANG W, KUMAR P, SCHWARZ M, MALONE G, HAWORTH A and KUMAR S: PCR AMPLIFICATION OF 40-YEAR-OLD PARAFFIN-EMBEDDED TUMOR-TISSUES - COMPARISON OF 4 DIFFERENT DNA EXTRACTION AND PURIFICATION METHODS. Int J Oncol 5: 453-457, 1994.
APA
WANG, W., KUMAR, P., SCHWARZ, M., MALONE, G., HAWORTH, A., & KUMAR, S. (1994). PCR AMPLIFICATION OF 40-YEAR-OLD PARAFFIN-EMBEDDED TUMOR-TISSUES - COMPARISON OF 4 DIFFERENT DNA EXTRACTION AND PURIFICATION METHODS. International Journal of Oncology, 5, 453-457. https://doi.org/10.3892/ijo.5.3.453
MLA
WANG, W., KUMAR, P., SCHWARZ, M., MALONE, G., HAWORTH, A., KUMAR, S."PCR AMPLIFICATION OF 40-YEAR-OLD PARAFFIN-EMBEDDED TUMOR-TISSUES - COMPARISON OF 4 DIFFERENT DNA EXTRACTION AND PURIFICATION METHODS". International Journal of Oncology 5.3 (1994): 453-457.
Chicago
WANG, W., KUMAR, P., SCHWARZ, M., MALONE, G., HAWORTH, A., KUMAR, S."PCR AMPLIFICATION OF 40-YEAR-OLD PARAFFIN-EMBEDDED TUMOR-TISSUES - COMPARISON OF 4 DIFFERENT DNA EXTRACTION AND PURIFICATION METHODS". International Journal of Oncology 5, no. 3 (1994): 453-457. https://doi.org/10.3892/ijo.5.3.453
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