ENHANCED EXPRESSION OF C-MYC GENE AND BINDING-PROTEIN TO THE 2.3-KB UPSTREAM REGION OF THE P1 PROMOTER IN HUMAN LIVER AND COLON CANCERS
- Authors:
- Published online on: November 1, 1994 https://doi.org/10.3892/ijo.5.5.1099
- Pages: 1099-1104
Metrics: Total
Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Abstract
We investigated binding proteins in the upstream regulatory region of c-myc gene in human liver and colon cancers. Gel shift assay revealed a nuclear protein which bound to the HindIII/PstI fragment (nt.-2328 to nt.-2118) upstream of the pi promoter of c-myc gene. DNase I footprinting showed a unique 25bp binding sequence (nt.-2305 to nt.-2281), not related to any reported elements. The amount of binding protein was higher in the cancerous than in the non-cancerous tissues. This suggests that the 25bp sequence possibly functions as a cis-acting positive element of the c-myc gene expression.