OVEREXPRESSION OF GLUCOSE-6-PHOSPHATE-DEHYDROGENASE IN RAT HEPATIC PRENEOPLASIA AND NEOPLASIA

  • Authors:
    • H STUMPF
    • P BANNASCH
  • View Affiliations

  • Published online on: December 1, 1994     https://doi.org/10.3892/ijo.5.6.1255
  • Pages: 1255-1260
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Abundance of messenger RNA (mRNA) and activity of glucose-6-phosphate dehydrogenase (G6PDH), the rate limiting enzyme of the oxidative pentose phosphate pathway, were studied in preneoplastic foci of altered hepatocytes (FAH) and hepatocellular carcinomas (HCC) induced in rats by limited oral exposure to N-nitrosomorpholine (NNM-stop model). Northern blot analysis revealed increased amounts of G6PDH-mRNA in 9/10 HCC, which are apparently not due to structural alterations of the G6PDH gene as studied by Southern blot analysis. In four additional HCC elevated expression of G6PDH was demonstrated by in situ hybridization to antisense-mRNA and by catalytic enzyme histochemistry. This correlative molecular genetic and enzyme histochemical approach was also used to study G6PDH expression in FAH of different phenotypes, namely glycogen storage foci (GSF), mixed cell foci (MCF) acid basophilic cell foci (BCF), representing early (GSF) and advanced (MCF, BCF) stages of hepatic preneoplasia. mRNA level and activity of G6PDH were closely correlated in all types of lesions and increased from GSF to MCF/BCF and HCC. These results suggest a predominantly transcriptional regulation of the increasing expression of G6PDH during hepatocarcinogenesis in the rat.

Related Articles

Journal Cover

December 1994
Volume 5 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
STUMPF H and STUMPF H: OVEREXPRESSION OF GLUCOSE-6-PHOSPHATE-DEHYDROGENASE IN RAT HEPATIC PRENEOPLASIA AND NEOPLASIA. Int J Oncol 5: 1255-1260, 1994
APA
STUMPF, H., & STUMPF, H. (1994). OVEREXPRESSION OF GLUCOSE-6-PHOSPHATE-DEHYDROGENASE IN RAT HEPATIC PRENEOPLASIA AND NEOPLASIA. International Journal of Oncology, 5, 1255-1260. https://doi.org/10.3892/ijo.5.6.1255
MLA
STUMPF, H., BANNASCH, P."OVEREXPRESSION OF GLUCOSE-6-PHOSPHATE-DEHYDROGENASE IN RAT HEPATIC PRENEOPLASIA AND NEOPLASIA". International Journal of Oncology 5.6 (1994): 1255-1260.
Chicago
STUMPF, H., BANNASCH, P."OVEREXPRESSION OF GLUCOSE-6-PHOSPHATE-DEHYDROGENASE IN RAT HEPATIC PRENEOPLASIA AND NEOPLASIA". International Journal of Oncology 5, no. 6 (1994): 1255-1260. https://doi.org/10.3892/ijo.5.6.1255