INDUCTION OF P-GLYCOPROTEIN, GLUTATHIONE-S-TRANSFERASE-PI, CATALASE, C-FOS AND C-ERBB1 IN RODENT CELL-LINES AFTER EXPOSURE TO DOXORUBICIN, ETHANOL AND CAFFEINE

  • Authors:
    • M VOLM
    • T EFFERTH
    • P GRABNER
    • EW POMMERENKE
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  • Published online on: January 1, 1995     https://doi.org/10.3892/ijo.6.1.87
  • Pages: 87-92
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Abstract

Murine NIH-3T3 cells were exposed to doxorubicin (DOX, 1 mu g/ml), ethanol (EtOH, 0.2%) and caffeine (CAFF, 200 mu g/ml) and analyzed for the induction of resistance proteins (P-glycoprotein, glutathione S-transferase-pi, catalase) and oncoproteins (c-EOS, c-ERBB1). P-glycoprotein (P-170), glutathione S-transferase-pi (GST-pi) and catalase (CAT) levels were found to be elevated after exposure of the cells to doxorubicin. In EtOH-treated cells the P-170 level was moderately increased (12 to 36 h after exposure), whereas the GST-pi and CAT levels were greatly increased (1 to 48 h). CAFF caused a moderate increase of P-170 (12 to 36 h) and of GST-pi (6 to 72 h). The accumulation of rhodamine 123 was reduced after the level of the resistance proteins had risen. After exposure to DOX, c-FOS was expressed moderately whereas c-ERBB1 was expressed strongly. Both oncoproteins showed a significant increase after exposure to EtOH. Only a moderate increase of c-FOS was seen after exposure to CAFF. Five out of seven additionally investigated rodent cell lines showed an increase in the expression of P-170, GST-pi and c-FOS after exposure to DOX, EtOH or CAFF.

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January 1995
Volume 6 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
VOLM M, EFFERTH T, GRABNER P and POMMERENKE E: INDUCTION OF P-GLYCOPROTEIN, GLUTATHIONE-S-TRANSFERASE-PI, CATALASE, C-FOS AND C-ERBB1 IN RODENT CELL-LINES AFTER EXPOSURE TO DOXORUBICIN, ETHANOL AND CAFFEINE. Int J Oncol 6: 87-92, 1995.
APA
VOLM, M., EFFERTH, T., GRABNER, P., & POMMERENKE, E. (1995). INDUCTION OF P-GLYCOPROTEIN, GLUTATHIONE-S-TRANSFERASE-PI, CATALASE, C-FOS AND C-ERBB1 IN RODENT CELL-LINES AFTER EXPOSURE TO DOXORUBICIN, ETHANOL AND CAFFEINE. International Journal of Oncology, 6, 87-92. https://doi.org/10.3892/ijo.6.1.87
MLA
VOLM, M., EFFERTH, T., GRABNER, P., POMMERENKE, E."INDUCTION OF P-GLYCOPROTEIN, GLUTATHIONE-S-TRANSFERASE-PI, CATALASE, C-FOS AND C-ERBB1 IN RODENT CELL-LINES AFTER EXPOSURE TO DOXORUBICIN, ETHANOL AND CAFFEINE". International Journal of Oncology 6.1 (1995): 87-92.
Chicago
VOLM, M., EFFERTH, T., GRABNER, P., POMMERENKE, E."INDUCTION OF P-GLYCOPROTEIN, GLUTATHIONE-S-TRANSFERASE-PI, CATALASE, C-FOS AND C-ERBB1 IN RODENT CELL-LINES AFTER EXPOSURE TO DOXORUBICIN, ETHANOL AND CAFFEINE". International Journal of Oncology 6, no. 1 (1995): 87-92. https://doi.org/10.3892/ijo.6.1.87