PCR DETECTION OF CLONAL TCR GAMMA-GENE REARRANGEMENTS IN A GROUP OF CUTANEOUS T-CELL LYMPHOMAS INCLUDING A CASE OF LOCALIZED PAGETOID RETICULOSIS EXPRESSING THE GAMMA-DELTA TCR

  • Authors:
    • M ALAIBAC
    • R YU
    • AC CHU
  • View Affiliations

  • Published online on: June 1, 1995     https://doi.org/10.3892/ijo.6.6.1267
  • Pages: 1267-1270
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Abstract

In this report, we describe the analysis of clonality performed using a PCR-based approach in a group of cutaneous T-cell lymphomas including a case of localised pagetoid reticulosis expressing the gamma delta T-cell receptor. For our PCR analysis DNA was extracted from frozen tissue of 10 cases of cutaneous T-cell lymphomas and subjected to polymerase chain reaction amplification using primers recognizing conserved sequences of the variable and joining regions of the T-cell receptor gamma chain gene. PCR products were then analyzed by high resolution non-denaturing polyacrylamide gel electrophoresis. Clonal TCR gamma gene rearrangements were detected in all the cases investigated including the case of localised pagetoid reticulosis. The PCR-based approach used in this study may provide an alternative to conventional Southern blot analysis for the analysis of cutaneous T-cell lymphomas as it is easy to perform and requires only minimal amounts of DNA. The findings in localized pagetoid reticulosis are consistent with the proliferation of monoclonal T-cells in lesional skin of this condition and provide evidence for the lymphoproliferative nature of this clinically benign disorder.

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June 1995
Volume 6 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
ALAIBAC M, YU R and CHU A: PCR DETECTION OF CLONAL TCR GAMMA-GENE REARRANGEMENTS IN A GROUP OF CUTANEOUS T-CELL LYMPHOMAS INCLUDING A CASE OF LOCALIZED PAGETOID RETICULOSIS EXPRESSING THE GAMMA-DELTA TCR. Int J Oncol 6: 1267-1270, 1995
APA
ALAIBAC, M., YU, R., & CHU, A. (1995). PCR DETECTION OF CLONAL TCR GAMMA-GENE REARRANGEMENTS IN A GROUP OF CUTANEOUS T-CELL LYMPHOMAS INCLUDING A CASE OF LOCALIZED PAGETOID RETICULOSIS EXPRESSING THE GAMMA-DELTA TCR. International Journal of Oncology, 6, 1267-1270. https://doi.org/10.3892/ijo.6.6.1267
MLA
ALAIBAC, M., YU, R., CHU, A."PCR DETECTION OF CLONAL TCR GAMMA-GENE REARRANGEMENTS IN A GROUP OF CUTANEOUS T-CELL LYMPHOMAS INCLUDING A CASE OF LOCALIZED PAGETOID RETICULOSIS EXPRESSING THE GAMMA-DELTA TCR". International Journal of Oncology 6.6 (1995): 1267-1270.
Chicago
ALAIBAC, M., YU, R., CHU, A."PCR DETECTION OF CLONAL TCR GAMMA-GENE REARRANGEMENTS IN A GROUP OF CUTANEOUS T-CELL LYMPHOMAS INCLUDING A CASE OF LOCALIZED PAGETOID RETICULOSIS EXPRESSING THE GAMMA-DELTA TCR". International Journal of Oncology 6, no. 6 (1995): 1267-1270. https://doi.org/10.3892/ijo.6.6.1267