SUPPRESSION AND RESTORATION OF V-SRC EXPRESSION IN RSV TRANSFORMED-CELLS AFTER TRANSFECTION WITH N-RAS AND ITS ANTAGONIST

  • Authors:
    • EM TCHEVKINA
    • NP KISSELJOVA
    • MS SHTUTMAN
    • EA MUSATKINA
    • OA MIZENINA
    • KV LESKOV
    • A TAVITIAN
    • FL KISSELJOV
  • View Affiliations

  • Published online on: September 1, 1995     https://doi.org/10.3892/ijo.7.3.453
  • Pages: 453-459
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Previously, it was shown that hamster cells transformed by Rous Sarcoma Virus (RSV) exhibited a decreased expression of the RSV products (including the pp60 src oncogene) when these cells were supertransfected with the N-ras oncogene. To assess the responsibility of the activated N-ras in the modulation of the RSV viral products, a strategy based on two ras antagonists was used; i.e. i) a rap1A/K-rev1 expression vector known for its capacity to revert the K-ras induced transformed phenotype and ii) a plasmid containing antisense N-ras sequence. We present data showing only the plasmid construct containing the N-ras antisense sequense could inhibit expression or N-ras and, at the same time, restore the expression of v-src, up to a level comparable to that of the parental cells. Our results support the idea that some biological switches, triggered and activated through the N-ras oncogene pathway, might modulate the promoter activity of the RSV LTR.

Related Articles

Journal Cover

September 1995
Volume 7 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
TCHEVKINA E, KISSELJOVA N, SHTUTMAN M, MUSATKINA E, MIZENINA O, LESKOV K, TAVITIAN A and KISSELJOV F: SUPPRESSION AND RESTORATION OF V-SRC EXPRESSION IN RSV TRANSFORMED-CELLS AFTER TRANSFECTION WITH N-RAS AND ITS ANTAGONIST. Int J Oncol 7: 453-459, 1995.
APA
TCHEVKINA, E., KISSELJOVA, N., SHTUTMAN, M., MUSATKINA, E., MIZENINA, O., LESKOV, K. ... KISSELJOV, F. (1995). SUPPRESSION AND RESTORATION OF V-SRC EXPRESSION IN RSV TRANSFORMED-CELLS AFTER TRANSFECTION WITH N-RAS AND ITS ANTAGONIST. International Journal of Oncology, 7, 453-459. https://doi.org/10.3892/ijo.7.3.453
MLA
TCHEVKINA, E., KISSELJOVA, N., SHTUTMAN, M., MUSATKINA, E., MIZENINA, O., LESKOV, K., TAVITIAN, A., KISSELJOV, F."SUPPRESSION AND RESTORATION OF V-SRC EXPRESSION IN RSV TRANSFORMED-CELLS AFTER TRANSFECTION WITH N-RAS AND ITS ANTAGONIST". International Journal of Oncology 7.3 (1995): 453-459.
Chicago
TCHEVKINA, E., KISSELJOVA, N., SHTUTMAN, M., MUSATKINA, E., MIZENINA, O., LESKOV, K., TAVITIAN, A., KISSELJOV, F."SUPPRESSION AND RESTORATION OF V-SRC EXPRESSION IN RSV TRANSFORMED-CELLS AFTER TRANSFECTION WITH N-RAS AND ITS ANTAGONIST". International Journal of Oncology 7, no. 3 (1995): 453-459. https://doi.org/10.3892/ijo.7.3.453