TUMORIGENICITY ASSOCIATED WITH CHROMOSOME 11P15 ALTERATIONS IN SV40-TRANSFORMED HUMAN KIDNEY-CELLS

  • Authors:
    • V POIRIER
    • SJ TYLER
    • J SLEEMAN
    • F DIFFIN
    • KW BROWN
  • View Affiliations

  • Published online on: September 1, 1995     https://doi.org/10.3892/ijo.7.3.623
  • Pages: 623-630
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Abstract

Chromosome 11p15 has been suggested to be a potential site for a second Wilms' tumour gene (a childhood nephroblastoma). Human foetal kidney cells and normal kidney cells from Wilms' tumour patients were transformed with SV40 derivative vectors. As some of the cell lines progressed to tumorigenicity, we observed that chromosome 11p13, site of the WT1 suppressor gene, did not show any allelic loss. However, RFLP analysis showed that chromosome 11p15 was affected by allelic losses on different genes in some cell lines but not necessarily prior to the appearance of tumorigenicity. We also observed that the most aggressive cell Line (SVCU/NK), derived from the normal kidney cells of a Wilms' tumour patient, showed increased expression of c-Ha-ras proto-oncogene at later passage and in the tumour tissue extracted from nude mice. Finally we report a lack of tumour suppression activity of one cell line SVT1B6/NK, when fused with the tumorigenic G401 cell line (the latter has been used in tumour suppression experiments as a Wilms' tumour cell line before being identified as a Rhabdoid tumour cell line). These experiments are consistent with the existence of a suppressor gene at chromosome 11p15.

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September 1995
Volume 7 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
POIRIER V, TYLER S, SLEEMAN J, DIFFIN F and BROWN K: TUMORIGENICITY ASSOCIATED WITH CHROMOSOME 11P15 ALTERATIONS IN SV40-TRANSFORMED HUMAN KIDNEY-CELLS. Int J Oncol 7: 623-630, 1995
APA
POIRIER, V., TYLER, S., SLEEMAN, J., DIFFIN, F., & BROWN, K. (1995). TUMORIGENICITY ASSOCIATED WITH CHROMOSOME 11P15 ALTERATIONS IN SV40-TRANSFORMED HUMAN KIDNEY-CELLS. International Journal of Oncology, 7, 623-630. https://doi.org/10.3892/ijo.7.3.623
MLA
POIRIER, V., TYLER, S., SLEEMAN, J., DIFFIN, F., BROWN, K."TUMORIGENICITY ASSOCIATED WITH CHROMOSOME 11P15 ALTERATIONS IN SV40-TRANSFORMED HUMAN KIDNEY-CELLS". International Journal of Oncology 7.3 (1995): 623-630.
Chicago
POIRIER, V., TYLER, S., SLEEMAN, J., DIFFIN, F., BROWN, K."TUMORIGENICITY ASSOCIATED WITH CHROMOSOME 11P15 ALTERATIONS IN SV40-TRANSFORMED HUMAN KIDNEY-CELLS". International Journal of Oncology 7, no. 3 (1995): 623-630. https://doi.org/10.3892/ijo.7.3.623