CROSS-SENSITIVITY TO X-RADIATION AND TYPE-I AND TYPE-II DNA TOPOISOMERASE INHIBITORS IN A RANGE OF HUMAN AND RODENT CELL-LINES

  • Authors:
    • CE NG
    • AM BUSSEY
    • HA MACDONALD
    • DP HELLER
    • DE WILKINS
    • GP RAAPHORST
  • View Affiliations

  • Published online on: November 1, 1995     https://doi.org/10.3892/ijo.7.5.1179
  • Pages: 1179-1184
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Abstract

We compared the relative X-radiation response of confluent (i.e. essentially non-proliferating) cultures of three human tumor (U-87MG, Mel-3, HT-144), one human normal (AG1522) and two rodent normal (AA8, V3) cell lines to their relative sensitivities to a DNA topoisomerase (topo) II poison (etoposide) and to a topo I poison (camptothecin). The relative sensitivity of these cell lines to etoposide (for 8 h exposure at 37 degrees C) is extremely similar to their relative X-radiation sensitivity, suggesting a direct correlation between X-radiation sensitivity and susceptibility to killing by topo II poison. The relative sensitivities of these cell lines to camptothecin (also 8 h, 37 degrees C exposure) also agree generally with their relative X-radiation sensitivities although the correlation is not as good as for etoposide. In addition, exponential phase (i.e, actively proliferating) cultures of the radiosensitive HT-144 cells are more susceptible to killing by both etoposide and camptothecin than the radioresistant Mel-3, confirming previously reported cross-sensitivities between X-radiation and topo poisons in actively proliferating cultures of other types of cell lines. Hence our results suggest that the previously reported cross-sensitivity between topo II poisons and X-radiation in actively proliferating rodent cell lines is also observed in 'non-proliferating' rodent and human cell lines. Additionally, there is cross-sensitivity between topo I poisons and X-radiation in both rodent and human cell lines as well.

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November 1995
Volume 7 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
NG C, BUSSEY A, MACDONALD H, HELLER D, WILKINS D and RAAPHORST G: CROSS-SENSITIVITY TO X-RADIATION AND TYPE-I AND TYPE-II DNA TOPOISOMERASE INHIBITORS IN A RANGE OF HUMAN AND RODENT CELL-LINES. Int J Oncol 7: 1179-1184, 1995
APA
NG, C., BUSSEY, A., MACDONALD, H., HELLER, D., WILKINS, D., & RAAPHORST, G. (1995). CROSS-SENSITIVITY TO X-RADIATION AND TYPE-I AND TYPE-II DNA TOPOISOMERASE INHIBITORS IN A RANGE OF HUMAN AND RODENT CELL-LINES. International Journal of Oncology, 7, 1179-1184. https://doi.org/10.3892/ijo.7.5.1179
MLA
NG, C., BUSSEY, A., MACDONALD, H., HELLER, D., WILKINS, D., RAAPHORST, G."CROSS-SENSITIVITY TO X-RADIATION AND TYPE-I AND TYPE-II DNA TOPOISOMERASE INHIBITORS IN A RANGE OF HUMAN AND RODENT CELL-LINES". International Journal of Oncology 7.5 (1995): 1179-1184.
Chicago
NG, C., BUSSEY, A., MACDONALD, H., HELLER, D., WILKINS, D., RAAPHORST, G."CROSS-SENSITIVITY TO X-RADIATION AND TYPE-I AND TYPE-II DNA TOPOISOMERASE INHIBITORS IN A RANGE OF HUMAN AND RODENT CELL-LINES". International Journal of Oncology 7, no. 5 (1995): 1179-1184. https://doi.org/10.3892/ijo.7.5.1179