Comparative analysis of p16/CDKN2, p53 and ras gene alterations in human non-small cell lung cancers, with and without associated pulmonary asbestosis

  • Authors:
    • I Hayashi
    • N Konishi
    • H Matsuda
    • T Tsuzuki
    • M Tao
    • Y Kitahori
    • T Tokuyama
    • T Yoneda
    • N Narita
    • Y Hiasa
  • View Affiliations

  • Published online on: January 1, 1996     https://doi.org/10.3892/ijo.8.1.85
  • Pages: 85-90
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

To investigate genetic abnormalities in human non-small cell lung carcinomas (NSCLC) associated with pulmonary asbestosis as compared with nou-asbestos linked lung cancers, twenty-nine primary non-small cell lung carcinomas (NSCLC) were examined for genetic abnormalities of p16/CDKN2, p53 and ras genes by single-strand conformation polymorphism analysis of polymerase chain reaction products (PCR-SSCP) and direct sequencing. Ten specimens were obtained from autopsies in which concurrent pulmonary asbestosis was present, while 19 samples were surgical specimens from asbestosis-free patients. K-ras mutations were detected in 10% each of the cancers from both asbestosis and non-asbestosis cases. p16/CDKN2 deletions or mutations and p53 aberrations were demonstrated in 20% and 10% of tumors from asbestosis cases, whereas, 32% and 21% of the cancers, respectively, from asbestosis-free patients were positive. In conclusion, it is suggested that the enhancement of neoplasia in the lung by asbestos is not dependent on suppression of p16/CDKN2 and p53 or ras activation and therefore, that asbestosis may activate alternate tumorigenic pathways in the development of NSCLC.

Related Articles

Journal Cover

January 1996
Volume 8 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Hayashi I, Konishi N, Matsuda H, Tsuzuki T, Tao M, Kitahori Y, Tokuyama T, Yoneda T, Narita N, Hiasa Y, Hiasa Y, et al: Comparative analysis of p16/CDKN2, p53 and ras gene alterations in human non-small cell lung cancers, with and without associated pulmonary asbestosis. Int J Oncol 8: 85-90, 1996
APA
Hayashi, I., Konishi, N., Matsuda, H., Tsuzuki, T., Tao, M., Kitahori, Y. ... Hiasa, Y. (1996). Comparative analysis of p16/CDKN2, p53 and ras gene alterations in human non-small cell lung cancers, with and without associated pulmonary asbestosis. International Journal of Oncology, 8, 85-90. https://doi.org/10.3892/ijo.8.1.85
MLA
Hayashi, I., Konishi, N., Matsuda, H., Tsuzuki, T., Tao, M., Kitahori, Y., Tokuyama, T., Yoneda, T., Narita, N., Hiasa, Y."Comparative analysis of p16/CDKN2, p53 and ras gene alterations in human non-small cell lung cancers, with and without associated pulmonary asbestosis". International Journal of Oncology 8.1 (1996): 85-90.
Chicago
Hayashi, I., Konishi, N., Matsuda, H., Tsuzuki, T., Tao, M., Kitahori, Y., Tokuyama, T., Yoneda, T., Narita, N., Hiasa, Y."Comparative analysis of p16/CDKN2, p53 and ras gene alterations in human non-small cell lung cancers, with and without associated pulmonary asbestosis". International Journal of Oncology 8, no. 1 (1996): 85-90. https://doi.org/10.3892/ijo.8.1.85